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目的:筛选参与口腔扁平苔藓(oral lichen planus,OLP)上皮细胞凋亡以及炎症反应相关的胞内关键转录信号蛋白。方法:选取OLP患者8例与正常对照者6例,分别收集口腔损害黏膜组织与正常颊黏膜组织及外周血5 m L,并提取各组黏膜组织与外周血单个核细胞的蛋白及测定蛋白浓度。使用奥德赛~■红外成像系统扫描并利用LI-COR~■ Image Studio v2.0芯片分析软件测量各组平均RFU值后统计分析。结果:与正常对照和萎缩糜烂型OLP组比较,网纹型OLP损害组织中p38 MAPK(Thr180/Tyr204)明显升高(P<0.001,P<0.001)。而STAT3(Ser727)在正常对照组、网纹型以及萎缩糜烂型OLP组织中的表达水平依次升高(P<0.001,P=0.002)。此外,在外周血中,与正常对照组比较,OLP患者p38 MAPK(Thr180/Tyr204)与STAT3(Ser727)表达均明显升高。结论:STAT3(Ser727)与p38 MAPK(Thr180/Tyr204)可能是OLP疾病发生发展过程中与临床分型密切相关的关键转录信号蛋白。
OBJECTIVE: To screen intracellular key transcriptional signaling proteins involved in the apoptosis of epithelial cells and inflammatory response in oral lichen planus (OLP). Methods: Eight OLP patients and 6 normal controls were selected. Oral mucosa tissues and normal buccal mucosa tissues and peripheral blood were collected for 5 m L, and the protein and protein concentration of mucosa and peripheral blood mononuclear cells . Using Odyssey ~ ■ infrared imaging system scan and use LI-COR ~ ■ Image Studio v2.0 chip analysis software to measure the average RFU value of each group after the statistical analysis. Results: Compared with the normal OLE group and the atrophic erosive OLP group, the p38 MAPK (Thr180 / Tyr204) in the OLP group was significantly increased (P <0.001, P <0.001). However, the expression of STAT3 (Ser727) in normal control group, anovulatory group and atrophic erosive OLP group increased in turn (P <0.001, P = 0.002). In addition, compared with the normal control group, the expression of p38 MAPK (Thr180 / Tyr204) and STAT3 (Ser727) in OLP patients were significantly increased in peripheral blood. CONCLUSIONS: STAT3 (Ser727) and p38 MAPK (Thr180 / Tyr204) may be key transcriptional signal proteins closely related to clinical classification during the development of OLP.