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目的分析实时荧光定量PCR检测胃癌组织Twist基因的临床意义。方法用实时荧光定量PCR的方法检测正常胃黏膜、胃癌原发灶、胃癌转移灶组织中TwistmRNA的表达含量。并以Twist基因和18 s tRNA含量的比值作为评价Twist表达水平的指标,组间进行配对t检验。结果Twist/18 s tRNA(log比值)正常组为0.139±0.003;原发组为0.304±0.046;转移组为0.654±0.015。原发性胃癌组织及转移组织中Twist基因表达水平与正常胃黏膜组织比较,差异有统计学意义(P<0.05);转移组与原发组比较,差异有统计学意义(P<0.05)。结论Twist基因在正常胃黏膜组织中表达量较低,在胃癌及其转移组织中表达水平较高,而且Twist基因的表达水平与胃癌转移程度存在一定相关性。可以辅助诊断和观察胃癌术后疗效。
Objective To analyze the clinical significance of real-time fluorescent quantitative PCR in detecting Twist gene in gastric cancer. Methods The expression of Twist mRNA in normal gastric mucosa, primary gastric cancer and metastatic gastric cancer tissue was detected by real-time fluorescence quantitative PCR. The ratio of Twist gene and 18 s tRNA content was used as an index to evaluate the expression of Twist. Paired t-test was used to compare the two groups. Results Twist / 18 s tRNA (log ratio) was 0.139 ± 0.003 in normal group, 0.304 ± 0.046 in primary group and 0.654 ± 0.015 in metastasis group. The expression of Twist gene in primary gastric cancer and metastasis was significantly higher than that in normal gastric mucosa (P <0.05). There was significant difference between metastasis group and primary group (P <0.05). Conclusion The expression of Twist gene is lower in normal gastric mucosa tissues and higher in gastric cancer and its metastatic tissues. The expression level of Twist gene is correlated with the metastasis of gastric cancer. Can help diagnose and observe the effect of postoperative gastric cancer.