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目的研究无血清条件下慢性粒细胞白血病(CML)细胞诱导分化生成树突状细胞的可行性。方法采用 IL-4,GM-CSF 和 TNF-α联合诱导培养 CML 白血病细胞,用流式细胞检测细胞表型和 MTT 法检测 DC 细胞刺激自体或
Objective To study the feasibility of dendritic cells induced by chronic myeloid leukemia (CML) cells under serum-free conditions. Methods CML leukemia cells were induced by IL-4, GM-CSF and TNF-α, the cell phenotypes were detected by flow cytometry and MTT assay was used to detect DC cells stimulated autologous or