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目的探讨慢性间歇性缺氧以及复氧对大鼠学习记忆功能及海马CA3区胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)表达的影响。方法24只SD大鼠,分为空白组(UC组)、慢性间歇性缺氧组(CIH组)和复氧组(RH组),CIH组建立慢性间歇性缺氧模型,RH组前4周同CIH组,后4周正常饲养,所有大鼠在模型建立后进行Morris水迷宫测试后检测大鼠海马CA3区GFAP的表达。结果①Morris水迷宫逃避潜伏期成绩:CIH组>RH组>UC组(P<0.01,P<0.05);②穿越平台次数及跨越目标象限时间占整个游泳的时间百分率的比较:UC组>RH组>CIH组(P<0.01,P<0.05);③海马CA3区GFAP阳性产物COD值比较:CIH组>RH组>UC组(P<0.01)。结论①慢性间歇性缺氧可引起大鼠学习记忆功能的下降,复氧后大鼠学习记忆功能有所提高;②大鼠学习记忆功能的下降可能与海马CA3区GFAP的变化有关。
Objective To investigate the effects of chronic intermittent hypoxia and reoxygenation on the learning and memory abilities and the expression of glial fibrillary acidic protein (GFAP) in hippocampal CA3 region of rats. Methods Twenty-four SD rats were divided into blank group (UC group), chronic intermittent hypoxia group (CIH group) and reoxygenation group (RH group). Chronic intermittent hypoxia model was established in CIH group. In the same CIH group, normal rats were fed after 4 weeks. All rats were examined by Morris water maze test after establishment of the model to detect the expression of GFAP in hippocampus CA3 area. Results ① Morris water maze escape latency results: CIH group> RH group> UC group (P <0.01, P <0.05); ② The number of times across the platform and time span across the target quadrant of the total swimming time percentage: UC group> RH group> CIH group (P <0.01, P <0.05). ③Comparison of GFAP positive product COD in hippocampal CA3 area: CIH group> RH group> UC group (P <0.01). Conclusion ① Chronic intermittent hypoxia can cause the decline of learning and memory in rats, and improve the learning and memory ability of rats after reoxygenation. The decreased learning and memory ability of rats may be related to the changes of GFAP in hippocampal CA3 area.