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作者用快速、准确、灵敏的HPLC法对血清中苯巴比妥、乙胺嗪、苯妥英及其代谢产物5-(4-羟基苯) -5-苯妥英(HPPH)进行了同时测定.仪器:112型溶剂交换泵.160型UV检测器(检测波长214nm).层析预柱用十八烷基硅烷(ODS)填充(45×4.6mm,id),层析柱用5μm的球形ODS填充(250×4.6mm,id).方法:取50μl含0.5μg 5-(4-甲苯基)-5-苯妥英(内标)的乙腈液加到50μl的血清或血浆中,搅拌10s,然后离心2min,将上清液转入另一试管中,再离心30s,使沉淀颗粒分离完全.取5～10μl的上清液注入
The authors determined the phenobarbital, diethylcarbamazine, phenytoin and their metabolites 5- (4-hydroxybenzene) -5-phenytoin (HPPH) in serum simultaneously with fast, accurate and sensitive HPLC method.METHODS: 112 Type solvent exchange pump Model 160 UV detector (detection wavelength 214 nm). The prepacked column was packed with octadecylsilane (ODS) (45 × 4.6 mm, id), and the column was packed with 250 μm spherical ODS × 4.6mm, id) .Methods: 50μl of acetonitrile containing 0.5μg 5- (4-tolyl) -5-phenytoin (internal standard) was added to 50μl of serum or plasma and stirred for 10s, then centrifuged for 2min, The supernatant was transferred to another tube and centrifuged again for 30s to complete the pellet separation.Using 5 to 10 μl of supernatant was injected