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与低温黑暗处理相比 ,低温弱光处理使黄瓜叶片的放氧活性显著降低 ,2℃黑暗处理 6.5h后黄瓜叶片的放氧活性降低到处理前的 66% ,2℃ 35μmol/(m2· s)处理6.5h后则降低到处理前的 - 50 %。研究结果显示 ,在 5℃低温条件下 1 0 0 μmol/(m2· s)光照使黄瓜发生下列变化 :叶片毫秒延迟发光的慢相强度显著降低 ;从叶片中提取的叶绿体的PS 及 PS +PS 的电子传递速率显著升高 ;加 NH4 Cl和加 ADP+Pi的 Hill反应活性与基础 Hill反应活性相差不大 ;从叶片中提取的叶绿体 9- AA荧光猝灭能力消失。以上结果证明低温下光照处理使黄瓜类囊体膜解耦联。
Compared with the low temperature darkness treatment, the oxygen release activity of cucumber leaves decreased significantly under low temperature and low light treatment. After the darkness treatment at 2 ℃ for 6.5h, the oxygen release activity of cucumber leaves decreased to 66% of the pre-treatment temperature, and 2 ℃ 35μmol / (m2 · s) ) Decreased to -50% before treatment after 6.5h treatment. The results showed that under the low temperature of 5 ℃, the changes of cucumber under the light of 100 μmol / (m2 · s) showed that the slow phase intensity of delayed luminescence was significantly reduced in milliseconds; the chloroplast PS and PS + PS The Hill reaction activity of NH4Cl plus ADP + Pi was similar to that of Hill reaction. The fluorescence quenching ability of chloroplast 9-AA extracted from leaves disappeared. The above results show that the light treatment at low temperature to cucumber thylakoid membrane decoupling.