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Based on the adsorption of copper ions on single-walled carbon nanotubes(SWNTs)in electrolyte,Cu/SWNTs nanocomposite film was initially prepared on indium-doped tin oxide(ITO)substrate by one-step electrodeposition.This method may provide a versatile and facile pathway to fabricate other SWNTs-supported metal composite films.Electrochemical experiments revealed that the obtained Cu/SWNTs/ITO electrode offered an excellent electrocatalytic activity towards the oxidation of glucose and could be applied to the construction of non-enzymatic glucose biosensor.The linear range of the sensor was 1.0× 10-6to 6.0× 10-4 mol/L and the response time was within 2 s.Particularly,its sensitivity reached as high as 1434.67 μA.L·mmol-1·cm-2,which was superior to any other non-enzymatic glucose biosensor based on copper-carbon nanotubes electrode reported previously.