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利用30,90,180Gy3种剂量的12C6+重离子束辐照大葱种子,研究其在细胞水平和农艺性状的诱变效应并进行RAPD分析。通过与M1代的研究结果比较后表明:经过不同剂量12C6+重离子照射后能有效地诱导大葱细胞形成微核和染色体畸变,这种诱变效应,在M2代仍然有所表现。M1代大葱结果期的株高、白长、花序直径和种子产量随辐照剂量增加产生明显差别,其中30Gy辐照组增幅最大。大葱总水溶性蛋白质和维生素C的含量在30Gy组中积累最多,在90Gy组有明显下降。与M1代一致,M2代中大葱染色体微核率及RAPD分析所得的DNA多态性比率仍然与辐照剂量呈正相关,但比率整体下降;说明高能量重离子辐照造成的DNA变异在M2代被修复和淘汰。
The seeds of Welsh onion were irradiated with 12,96,180Gy doses of 12C6 + heavy ion beams to study their mutagenic effects at cell level and agronomic traits and RAPD analysis. Compared with the results of M1 generation, it was shown that the formation of micronuclei and chromosome aberration induced by 12C6 + heavy ion irradiation of different doses could still be induced effectively in M2 generation. The results showed that the plant height, white length, inflorescence diameter and seed yield of M1 generation of green onions were significantly different with the increase of irradiation dose, and the highest increase occurred in 30Gy irradiation group. The content of total water-soluble protein and vitamin C in the shallot accumulated most in the 30Gy group and obviously decreased in the 90Gy group. In line with the M1 generation, the chromosome DNA micronucleus rate of M2 and the DNA polymorphism ratio of RAPD analysis were still positively correlated with the radiation dose, but the ratio decreased as a whole; that DNA mutation caused by high-energy heavy ion irradiation in the M2 generation Be repaired and eliminated.