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探索灵芝在以蕨渣为主要成分的固态基质中的培养条件,可为蕨渣的开发利用提供理论依据。以蕨渣为主要原料,采用响应面法对灵芝培养条件(基质蕨渣比例、基质含水量和培养温度)进行优化。结果表明,基质蕨渣比例、基质含水量和培养温度对灵芝菌丝日平均生长速率均有极显著的影响(p<0.01),且基质含水量与培养温度之间、基质蕨渣比例与基质含水量之间存在交互作用。优化出灵芝培养条件为蕨渣比例85%,基质含水量62.5%,培养温度27℃,在此条件下,灵芝菌丝日平均生长速率为3.48mm/d。多元回归分析结果显示,基质蕨渣比例、基质含水量、培养温度与菌丝日平均生长速率之间回归模型高度显著,可用于实际生产预测。首次报道了利用蕨渣培养灵芝,为蕨渣进一步的开发研究奠定基础。
To explore Ganoderma lucidum fern residue as the main component of the solid substrate in the culture conditions, fern residue can provide a theoretical basis for the development and utilization. Using fern residue as the main raw material, the response surface method was used to optimize the culture conditions of Ganoderma lucidum (ratio of matrix fern residue, substrate water content and incubation temperature). The results showed that the ratio of matrix fern residue, matrix water content and culture temperature had a significant effect on the average daily growth rate of G. lucidum (p <0.01), and the ratio of matrix fern residue to matrix Interactions exist between water contents. Optimized Ganoderma lucidum culture conditions for fern residue ratio of 85%, substrate moisture content of 62.5%, culture temperature 27 ℃, under these conditions, the average growth rate of Ganoderma lucidum mycelium 3.48mm / d. Multiple regression analysis showed that the regression model between the proportion of matrix fern residue, substrate water content, culture temperature and daily average growth rate of mycelium was highly significant and could be used for actual production prediction. For the first time reported using fern dregs to cultivate Ganoderma lucidum slag for further research and development and lay the foundation.