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目的研究特异性免疫治疗(SIT)对哮喘大鼠肺组织维甲酸相关孤核受体γt(RORγt)mRNA表达的影响及其机制。方法将24只正常清洁级雄性SD大鼠随机分成正常对照组(C组)、哮喘组(A组)和特异性免疫治疗组(SIT组),A组和SIT组通过卵白蛋白(OVA)致敏建立哮喘大鼠模型,SIT组再行OVA雾化吸入减敏。采用酶联免疫吸附试验(ELISA)检测大鼠支气管肺泡灌洗液(BALF)中白细胞介素-17(IL-17)及转化生长因子β1(TGF-β1)水平,采用实时荧光定量PCR方法检测肺组织RORγt mRNA表达水平。结果三组IL-17、TGF-β1和RORγt mRNA差异均有统计学意义(P<0.05)。A组IL-17、TGF-β1及RORγt mRNA均高于C组(P<0.05);SIT组IL-17及TGF-β1均高于C组(P<0.05),RORγt mRNA与C组差异无统计学意义(P>0.05);SIT组IL-17和TGF-β1及RORγt mRNA均低于A组(P<0.05)。RORγt mRNA与TGF-β1及IL-17均呈正相关(P<0.05)。结论 SIT可能通过减少TGF-β1分泌抑制RORγt表达,进而抑制Th17生成及IL-17分泌,减轻气道炎症反应。
Objective To investigate the effect of specific immunotherapy (SIT) on the mRNA expression of retinoic acid-related orphan nuclear receptor γt (RORγt) in the lung tissue of asthmatic rats and its mechanism. Methods Twenty-four normal male SD rats were randomly divided into normal control group (C group), asthma group (A group) and specific immunotherapy group (SIT group). A group and SIT group were induced by ovalbumin (OVA) Min Asthma rat model was established, SIT group followed by inhalation of OVA desensitization. The levels of interleukin-17 (IL-17) and transforming growth factor-β1 (TGF-β1) in bronchoalveolar lavage fluid (BALF) of rats were detected by enzyme linked immunosorbent assay (ELISA) Lung tissue RORγt mRNA expression levels. Results The differences of IL-17, TGF-β1 and RORγt mRNA in the three groups were statistically significant (P <0.05). The mRNA expressions of IL-17, TGF-β1 and RORγt in group A were significantly higher than those in group C (P <0.05). The levels of IL-17 and TGF-β1 in group SIT were significantly higher than those in group C Statistical significance (P> 0.05). The levels of IL-17, TGF-β1 and RORγt mRNA in SIT group were lower than those in A group (P <0.05). RORγt mRNA was positively correlated with TGF-β1 and IL-17 (P <0.05). Conclusion SIT may inhibit the expression of RORγt by decreasing the secretion of TGF-β1, and then inhibit the production of Th17 and the secretion of IL-17, and reduce the airway inflammation.