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目的:观察哈萨克族高血压患者外周血淋巴细胞白细胞介素6(IL-6)和其上游信号T细胞核因子(NFAT)以及电压门控钾通道Kv1.3的表达情况,以明确淋巴细胞在高血压发生发展中的作用。方法:通过随机数字表法随机选取20例首次就诊且未经药物治疗的新疆哈萨克族高血压病患者作为高血压组,20例哈萨克族健康体检者作为对照组。每位入选者均采肘静脉血10ml,用密度梯度离心法分离出单个核细胞,再采用免疫磁珠法分离外周血T淋巴细胞。用逆转录聚合酶链反应法(RT-PCR)检测两组间mRNA的拷贝数,并用Western blot检测对应蛋白的表达量。结果:高血压组的IL-6[(1.04±0.52)︰(0.21±0.04)]、NFATC1[(6.08±0.66)︰(1.04±0.26)]和Kv1.3[(1.99±0.50)︰(0.96±0.40)]的mRNA表达量均高于对照组(均P<0.05);且高血压组的IL-6[(0.63±0.02)︰(0.19±0.03)]、NFATC1[(0.45±0.03)︰(0.27±0.05)]的蛋白表达量也明显高于对照组(均P<0.05)。结论:Kv1.3可能通过激活钙调磷酸酶/活化的NFAT信号通道引起炎性细胞释放,从而参与哈萨克族高血压疾病的发生。
Objective: To observe the expression of interleukin-6 (IL-6) and its upstream signal T-cell nuclear factor (NFAT) and voltage-gated potassium channel Kv1.3 in Kazakh patients with hypertension in order to clarify the role of lymphocytes in high The role of blood pressure in the development. Methods: Twenty randomly selected Kazakh patients with hypertension in Xinjiang Uygur Autonomous Region who were treated for the first time without treatment with random number table were selected as hypertension group and 20 healthy Kazak people as control group. Each participant took elbow venous blood 10ml, isolated mononuclear cells by density gradient centrifugation, and then separated the peripheral blood T lymphocytes by immunomagnetic beads method. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the mRNA copy number between the two groups, and the corresponding protein was detected by Western blot. Results: The levels of IL-6 [(1.04 ± 0.52) :( 0.21 ± 0.04)], NFATC1 [(6.08 ± 0.66) :( 1.04 ± 0.26)] and Kv1.3 [(1.99 ± 0.50) (0.63 ± 0.02) :( 0.19 ± 0.03)], NFATC1 [(0.45 ± 0.03)) in hypertensive group were significantly higher than those in control group (all P <0.05) (0.27 ± 0.05)] were also significantly higher than those in the control group (all P <0.05). Conclusion: Kv1.3 may be involved in the occurrence of hypertension in Kazakhs through the activation of calcineurin / activated NFAT signaling pathway to release inflammatory cells.