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目的:观察叶黄素(LU)对顺铂(CP)诱导的大鼠急性肝损伤的保护作用。方法:将30只SD大鼠随机分为5组,分别为对照组、顺铂组、不同剂量叶黄素(10mg/kg、20mg/kg和40mg/kg)+顺铂组。大鼠连续灌胃叶黄素10天,第7天灌胃后l h腹腔注射顺铂(5mg/kg)。顺铂处理后,在第5天采血,测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)。采血后处死动物,测定肝脏系数、肝匀浆丙二醛(MDA)、一氧化氮(NO)、还原型谷胱甘肽(GSH)含量和超氧化物歧化酶(SOD)活力,同时观察肝组织结构变化。结果:肝损伤模型组大鼠血清ALT、AST含量均明显高于对照组,肝组织匀浆中MDA及NO含量显著增加,而GSH含量及SOD活性显著下降。叶黄素可降低肝损伤大鼠血清ALT及AST水平,降低肝组织匀浆MDA及NO水平,提高组织GSH含量及SOD活性,并可改善顺铂损伤大鼠肝病理组织学变化,随着叶黄素剂量增加,其保护作用增强。结论:叶黄素通过抗氧化作用减轻顺铂诱导的大鼠肝损伤作用。
Objective: To observe the protective effect of lutein (LU) on acute liver injury induced by cisplatin (CP) in rats. Methods: Thirty SD rats were randomly divided into 5 groups: control group, cisplatin group, different doses of lutein (10mg / kg, 20mg / kg and 40mg / kg) + cisplatin group. Rats were continuously fed with lutein for 10 days and intraperitoneally injected with cisplatin (5 mg / kg) on the 7th day after intragastric administration. After cisplatin treatment, blood was collected on the fifth day, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured. The animals were sacrificed after the blood was taken and the liver coefficient, malondialdehyde (MDA), nitric oxide (NO), glutathione (GSH) and superoxide dismutase (SOD) Changes in organizational structure. Results: The levels of serum ALT and AST in liver injury model group were significantly higher than those in control group. The content of MDA and NO in liver homogenate increased significantly, while the content of GSH and the activity of SOD decreased significantly. Lutein can reduce serum levels of ALT and AST, reduce MDA and NO in liver homogenate, increase GSH content and SOD activity in liver tissue, and improve histopathological changes of liver in cisplatin- Flavoprotein dose increased, its protective effect is enhanced. Conclusion: Lutein can reduce cisplatin-induced hepatic injury in rats through antioxidation.