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目的 :探讨 p16、Rb、cyclinD1和EGFR在星形细胞肿瘤发生、发展中的作用及其与预后的关系。 方法 :免疫组化染色采用即用型S P法 ,用图像分析系统对 80例星形细胞肿瘤瘤细胞进行DNA含量测定。结果 :80例星形细胞肿瘤 :p16、Rb、cyclinD1和EGFR的阳性率分别为 43 8%、71 3%、5 5 0 %和 85 0 % ,4种蛋白阳性率及DNA含量与组织学分级均有统计学意义 ;p16、Rb、cyclinD1阳性率和瘤细胞DNA含量与预后有关 ,EGFR阳性率与预后无关 ;Rb失表达时 ,p16表达增强。 结论 :p16和Rb在抑制星形细胞肿瘤的生长及EGFR和cyclinD1促使其生长中起着重要作用 ,p16表达同DNA含量相结合评估星形细胞瘤预后有一定价值。
Objective: To investigate the role of p16, Rb, cyclinD1 and EGFR in the development and progression of astrocytic tumors and its relationship with prognosis. Methods: Immunohistochemical staining method was used to determine the DNA content of 80 cases of astrocytoma cells by image analysis system. Results: The positive rates of p16, Rb, cyclinD1 and EGFR in 80 cases of astrocytic tumors were 43.8%, 71.3%, 55.0% and 85.0%, respectively. The positive rates of 4 proteins and the histological grade The positive rate of p16, Rb, cyclinD1 and tumor cell DNA content were related to the prognosis. The positive rate of EGFR had no correlation with the prognosis. The expression of p16 was increased when Rb was lost. CONCLUSION: p16 and Rb play an important role in inhibiting the growth of astrocytic tumors and promoting their growth by EGFR and cyclinD1. The combination of p16 expression and DNA content has some value in the prognosis of astrocytoma.