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薏苡(Coix lachryma-jobi)是一种药食兼用的作物,干旱是限制薏苡种植和生长最为主要的因素。为探讨烯效唑(uniconazole,S3307)在薏苡苗期抗旱方面的应用效果,本研究采用营养液水培法,以薏辽5号为材料,研究在温室条件下,外源S3307对干旱胁迫下薏苡生长指标、抗氧化酶活性、叶绿体含量及超微结构的影响。结果表明,9 mg/L S3307显著提高了干旱胁迫下薏苡幼苗的茎粗和生物量(P<0.05)。茎粗比干旱组(S0)提高了16.19%,地上、地下生物量比S0分别提高了19.56%和22.75%。另外,9mg/L S3307组中,过氧化物酶(peroxidase,POD),过氧化氢酶(catalase,CAT)和超氧化物歧化酶(superoxide dismutase,SOD)活性显著增加(P<0.05),其中根系的抗氧化酶活性要显著高于叶片(P<0.05)。在所有处理组中,9 mg/L S3307组中叶绿体含量最高,比干旱组增加7.35%(P<0.05)。同时,S3307能够减轻干旱胁迫下对叶片和根系细胞超微结构的影响;9 mg/L S3307能够有效减轻干旱胁迫对薏苡幼苗的伤害。研究结果为明确S3307缓解薏苡抗旱能力的生理机制提供了基础资料,同时为S3307在薏苡抗性生产中的应用提供理论依据。
Coix lachryma-jobi is a kind of crop used both for food and medicine. Drought is the most important factor that limits the planting and growth of Coix lachryma-jobi. In order to explore the application effect of uniconazole (S3307) on the drought resistance of seedling of Coix lachryma-jobi (Coix lachrymallotus), this study used nutrient solution hydroponics to study the effects of exogenous S3307 on drought resistance under greenhouse conditions Effects of Growth Index, Antioxidant Enzyme Activity, Chloroplast Content and Ultrastructure of Job’s tears Coix. The results showed that 9 mg / L S3307 significantly increased the stem diameter and biomass of seedlings (P <0.05) under drought stress. Compared with the drought group (S0), the stem diameter increased by 16.19% and the aboveground and underground biomass increased by 19.56% and 22.75% respectively compared with that of S0. In addition, the activities of peroxidase (POD), catalase (CAT) and superoxide dismutase (SOD) in 9mg / L S3307 group were significantly increased (P <0.05) The root antioxidant enzyme activity was significantly higher than the leaves (P <0.05). Among all treatment groups, the content of chloroplast in 9 mg / L S3307 group was the highest, 7.35% higher than that in drought group (P <0.05). At the same time, S3307 could alleviate the effects of drought stress on the ultrastructure of leaves and root cells; 9 mg / L S3307 could effectively reduce the damage of drought stress on the seedlings of Coix lachryma-jobi. The results provided the basic information for clarifying the physiological mechanism of S3307 alleviating the drought-resistant ability of Job’s tears and providing the theoretical basis for the application of S3307 in the resistant production of Job’s tears.