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背景 D抗原是高度免疫原性的人类红细胞抗原。对抗D抗原同种免疫产生高亲和力抗体,这些抗体可引起溶血性输血反应和新生儿溶血病。研究设计和方法 为了确定V(D)J种属基因的用途,对11例单克隆抗D的新样本进行克隆并随之作序列分析,并同以前已公布了的37例鉴定为V(H)和V(L)双链正侧结构、限制性种系基因构象序列的抗D样本进行分析和比较。结果 使用新的单克隆D抗体的V(H)和V(L)双链结构显示:
Background D antigen is a highly immunogenic human erythrocyte antigen. Alloimmunization against the D antigen produces high affinity antibodies that can cause hemolytic transfusion reactions and neonatal hemolytic disease. RESEARCH DESIGN AND METHODS In order to determine the use of the V (D) J genus, 11 new monoclonal anti-D samples were cloned and subsequently sequenced, along with 37 previously published V (H ) And V (L) double-stranded positive side structure, restriction gene conformation sequence of anti-D samples were analyzed and compared. Results The V (H) and V (L) double-stranded structures using the new monoclonal D antibodies showed: