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目的:观察刺山柑乙醇提取物乳膏对系统性硬皮病(SSc)小鼠外周血循环内皮细胞(CEC)数、血清一氧化氮(NO)及一氧化氮合酶(NOS)水平的影响。方法:BALB/c小鼠背部皮下注射盐酸博莱霉素诱导SSc模型,给予刺山柑乙醇提取物乳膏225,450,900 mg·kg~(-1)后,密度梯度离心法测定CEC数,硝酸还原酶法和ELISA法测定小鼠血清NO和NOS含量。结果:刺山柑乙醇提取物乳膏高剂量和阳性药青霉胺可降低小鼠CEC数,升高血清NO和NOS水平(P<0.01)。结论:刺山柑乙醇提取物乳膏对SSc血管内皮细胞损伤,NOS与NO释放减少有一定抑制作用。
Objective: To observe the effects of capparis ethanol extract cream on the number of circulating endothelial cells (CEC), serum nitric oxide (NO) and nitric oxide synthase (NOS) in systemic scleroderma mice (SSc) . Methods: BALB / c mice were inoculated subcutaneously with bleomycin hydrochloride to induce SSc model. After 225, 450, and 900 mg · kg -1 of capparisol extract were applied, density gradient centrifugation was used to determine the number of CEC, nitrate reductase The contents of NO and NOS in serum were measured by ELISA and ELISA. RESULTS: High dose and positive drug penicillamine could decrease the CEC number and increase the level of NO and NOS in serum (P <0.01). Conclusion: Capparis Ethanol extract cream can inhibit the injury of SSc vascular endothelial cells and decrease the release of NOS and NO.