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目的:探讨不同煎煮方法的麻杏石甘汤含药血清体外抗A型流感病毒的作用。方法:应用体外细胞培养技术进行狗肾细胞(MDCK)培养,通过3种不同的加药方式,检测不同煎煮方法的麻杏石甘汤含药血清对A型流感病毒直接灭活、干预吸附、抑制增殖的作用。结果:在直接灭活作用中,浓度为12.5%的麻黄先煎30 min组、先煎35 min组、先煎40 min组比同浓度四药同煎组具有更强的灭活流感病毒作用(P<0.05),其中麻黄先煎30 min组灭活能力最强(P<0.01);在干预吸附作用中,浓度为12.5%的麻黄先煎20、30、35 min组比四药同煎组对病毒吸附的干预作用强(P<0.05),其中麻黄先煎20、30 min组效果突出(P<0.01);在抑制增殖作用中,浓度为25%的麻黄先煎10 min组、20 min组、25 min组、30 min组、40 min组与四药同煎组相比较,对流感病毒的增殖抑制效果明显(P<0.05),其中先煎10、20、30 min效果突出(P<0.01)。上述3种作用都未体现出量效关系。结论:麻杏石甘汤煎煮方法的不同可以导致其抗流感病毒效果的差异。
Objective: To investigate the effect of Maxingshigan Decoction containing different decoction methods on anti-influenza A virus in vitro. Methods: MDCK cells were cultured in vitro. Three different dosing methods were used to detect the direct inactivation of the A-type influenza virus by Maxingshigan decoction-containing serum by different decoction methods, , Inhibit the role of proliferation. Results: In direct inactivation, ephedra at a concentration of 12.5% was decocted for 30 min and then decocted for 35 min before decoction. The decoction of 40 min had stronger inactivation of influenza virus than the same concentration of decoction P <0.05). The highest inactivation ability was observed after 30 min of Ephedra Decoction (P <0.01). In the intervening adsorption experiment, Ephedra Decoction of 20, 30, and 35 min at a concentration of 12.5% (P <0.05). The effect of Ephedrae Decoction for 20,30 min was prominent (P <0.01). In the inhibitory effect of proliferation, the Ephedra Decoction of 10% (P <0.05). The effect of inhibiting the proliferation of influenza virus was obvious at 25 min, 30 min and 40 min (P < 0.01). None of these three effects have shown a dose-response relationship. Conclusion: The different methods of Maxing Shigan Decoction can lead to the difference of anti-influenza virus effect.