用成年雄性Wistar大鼠,经4％多聚甲醛灌注固定,取下丘脑组织,行再固定24h,常规石蜡包埋和切片,用地高辛标记的cRNA探针进行原位杂交,结果显示在弓状核及其邻近区域见到阳性神经元,阳性信号为蓝色颗粒,胞质、核膜及核仁呈阳性。阴性对照均无阳性反应出现。本实验证实,用石蜡切片进行原位杂交组织化学研究,能检测下丘脑生长抑素神经元基因表达产物mRNA,方法稳定,重复性好,便于普通实验室开展工作。 Adult male Wistar rats were perfused with 4% paraformaldehyde, the thalamic tissues were removed and fixed for 24 hours. Paraffin-embedded and sectioned with paraffin and labeled with digoxigenin in situ hybridization. The results showed that in the bow Positive nuclei and their adjacent areas to see the positive neurons, positive signals for the blue particles, the cytoplasm, nuclear membrane and nucleolar positive. Negative control no positive reaction occurred. This experiment confirmed that paraffin sections for in situ hybridization histochemical studies can detect hypothalamic somatostatin neuron gene expression product mRNA, stable, reproducible, and easy to general laboratory work.