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目的为解决内源性感染细菌体内示踪问题，本实验采用ＰＵＣ１９质粒载体示踪加限制性内切酶酶切图谱分析法和荧光标记示踪法，对肠道移位细菌进行了动态观察。并对两种方法进行了比较。方法依据ＰＵＣ１９质粒特点设计了动物模型，１１０只Ｗｉｓａｒ大鼠致成３０％体表面积Ⅲ°烫伤，于６、１２、２４、４８ｈ和１２天分别活杀，做荧光显微镜检查和细菌培养，质粒提取和酶切。结果烧伤后肠道细菌可移位至淋巴结和肝脏，荧光标记方法细菌到达淋巴结（９５％）和肝脏（５７．５％）的百分率略高于ＰＵＣ１９示踪方法（７８．４％、５２．４％）。结论用ＰＵＣ１９质粒载体做示踪物可减少非特异性因素，为研究细菌引起内源性感染的发病机制提供了一种较为理想的方法
Objective To solve the tracing problem of endogenous infected bacteria in vivo, this study used PUC19 plasmid vector tracer plus restriction enzyme digestion mapping method and fluorescent labeling tracer method to observe the intestinal translocation bacteria. The two methods are compared. Methods The animal model was designed according to the characteristics of PUC19 plasmid. 110 Wisar rats were scalded for 30% body surface area Ⅲ ° for 5, 6, 12, 24, 48 and 12 days respectively. Fluorescent microscopy and bacterial culture were performed. Plasmid extraction And digested. Results Bacteria after trauma were able to translocate to the lymph nodes and liver. The percentage of bacteria reaching the lymph nodes (95%) and liver (57.5%) by fluorescence labeling method was slightly higher than that of the PUC19 tracer method (78.4%, 52.4 %). Conclusion The use of PUC19 as a tracer can reduce the nonspecific factors and provide a more ideal method for studying the pathogenesis of bacterial-induced endogenous infection