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目的探讨黄芪多糖对雄性肥胖小鼠精子蛋白酪氨酸磷酸酶1B(protein tyrosine phosphatase 1B,PTP1B)表达和顶体反应的影响。方法 40只C57BL/6J雄性小鼠随机分为对照组10只和高脂饲料组30只,分别给予普通饲料和高脂饲料喂养。8周后选取造模成功的肥胖小鼠20只,随机分为模型组和黄芪多糖(astragalus polysaccharides,APS)干预组(APS干预组),每组10只。对照组、模型组给予生理盐水灌胃,APS干预组给予APS 200mg/(kg·d)灌胃。4周后3组小鼠采用Western blot法检测精子PTP1B表达水平,采用金霉素染色法检测精子顶体反应率。结果高脂饲料组小鼠体质量[(32.8±1.7)g]明显高于对照组[(25.6±0.6)g](P<0.01);模型组小鼠精子顶体反应率[(10.6±6.2)%]明显低于对照组[(44.7±10.5)%]和APS干预组[(21.3±8.5)%](P<0.01),PTP1B表达水平(1 130.4±149.4)明显高于对照组(330.3±72.8)和APS干预组(485.9±85.6)(P<0.01)。结论 APS可改善肥胖小鼠精子顶体反应,其机制可能与其降低精子PTP1B有关。
Objective To investigate the effect of Astragalus Polysaccharides (APS) on sperm protein tyrosine phosphatase 1B (PTP1B) expression and acrosome reaction in obese mice. Methods Forty C57BL / 6J male mice were randomly divided into control group (n = 10) and high fat diet group (n = 30), which were fed with normal diet and high fat diet respectively. Twenty healthy obese mice were selected after 8 weeks and were randomly divided into model group and APS intervention group (APS intervention group), with 10 mice in each group. The control group and the model group were given gavage with saline, and the APS intervention group was given APS 200 mg / (kg · d). After 4 weeks, the three groups of mice were used to detect the expression of PTP1B by Western blot, and the acrosome reaction rate was determined by aureomycin staining. Results The body weight of mice in high fat diet group was significantly higher than that in control group [(25.6 ± 0.6) g] (P <0.01). The acrosome reaction rate in model group was (10.6 ± 6.2) ) Was significantly lower than that in the control group [(44.7 ± 10.5)%] vs [21.3 ± 8.5%] in the APS intervention group (P <0.01) ± 72.8) and APS intervention group (485.9 ± 85.6) (P <0.01). Conclusion APS can improve sperm acrosome reaction in obese mice, and its mechanism may be related to the reduction of sperm PTP1B.