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背景:在瘢痕研究中急需稳定可靠的实验动物模型。目的:运用组织学方法对裸鼠瘢痕动物模型进行鉴定,确定最佳使用时机。设计:随机对照的重复测量设计。单位:中山大学附属第一医院烧伤科。材料:实验于2004-01/03在中山大学医学院动物实验中心完成。15只4～6周龄裸鼠(性别随机,体质量15～25g)由中山大学医学院实验动物中心提供;增生性瘢痕来自烧伤愈后、瘢痕增生半年之患者手术切除标本。方法:于裸鼠背侧移植人增生性瘢痕,建立疤痕动物模型;移植后4周起,每周处死5只实验动物,取移植物,100g/L甲醛固定标本,持续3周。苦味酸-天狼猩红染色偏振光检测移植物及临床取材,观察组织特点。主要观察指标:①苦味酸-天狼猩红染色偏振光法读片结果。②计算机图像分析结果。结果:①苦味酸-天狼猩红染色偏振光法读片结果:各时段组移植物在偏振光下呈现相同的以黄、红粗大纤维为主、疏网状绿色纤细纤维散在分布的特点。②计算机图像分析结果:临床病理性增生性瘢痕中,Ⅰ型胶原约占74%,Ⅲ型胶原约占26%;4～6周移植物中,Ⅰ型胶原含量分别为(74.52±0.47)%,(74.43±0.53)%,(74.69±0.63)%;Ⅲ型胶原分别约为(25.48±0.47)%,(25.57±0.53)%,(25.31±0.63)%,差异不显著(P>0.05)。结论:在实验所设计时段中,移植物与临床取材特性一致,符合增生性瘢痕特点。苦味酸-天狼猩红染色偏振光检测瘢痕组织胶原是对增生性瘢痕组织测定的简便、有效手段;运用裸鼠建立瘢痕动物模型是行之有效的、稳定的。
Background: There is an urgent need for a stable and reliable experimental animal model in scar studies. OBJECTIVE: To identify the best animal model of scarring in nude mice by histological method. Design: Randomized, repeatable measurement design. Unit: First Affiliated Hospital of Sun Yat-sen Department of Burn. Materials: The experiment was performed at Animal Experimental Center of Sun Yat-sen Medical College from January to March 2004. Fifteen nude mice aged 4 to 6 weeks (random sex, body mass 15 ~ 25g) were provided by Laboratory Animal Center, School of Medicine, Sun Yat-sen University. Hypertrophic scars were obtained from patients who had burned more than half a year after scars. Methods: Hypertrophic scars were transplanted on the dorsal side of nude mice. Animal models of scar were established. Four weeks after transplantation, five experimental animals were sacrificed every week, and 100g / L formaldehyde fixed specimens were obtained for 3 weeks. Picric acid - Sirius red staining polarized light detection of graft and clinical material, observe the characteristics of tissue. MAIN OUTCOME MEASURES: ① picric acid - Sirius red staining polarized light reading results. ② computer image analysis results. Results: (1) Picric acid - Sirius red stained Polarized light reading results: the grafts under the polarized light showed the same characteristics of yellow and red coarse fibers, sparsely distributed network of sparse reticular green fibers. Results of computer image analysis: Type Ⅰ collagen accounted for about 74% and type Ⅲ collagen accounted for about 26% in clinical pathological hypertrophic scars. The content of type Ⅰ collagen was (74.52 ± 0.47)% , (74.43 ± 0.53)%, (74.69 ± 0.63)%, respectively. The type Ⅲ collagen was about (25.48 ± 0.47)%, (25.57 ± 0.53)% and (25.31 ± 0.63)%, . Conclusion: During the design period of the experiment, the characteristics of graft and clinical material were the same, which accorded with the characteristics of hypertrophic scars. Picric acid - Sirius red stained polarized light detection of scar tissue collagen hypertrophy scar tissue measurement is a simple and effective means; the use of nude mice to establish a scar animal model is effective and stable.