论文部分内容阅读
血浆样品用二氯甲烷提取，采用ＯＤＳ柱，以甲醇－磷酸盐缓冲液－三乙胺（３０：６９．８：０．２）为流动相，炎痛喜康为内标，２５４ｎｍ为检测波长，ＲＰ－ＨＰＬＣ法测定兔口服替诺昔康片后的体内血药浓度。线性范围０．１～３０μｇ／ｍｌ，最低检测用０．１μｇ／ｍｌ（Ｓ／Ｎ＞２）．萃取回收率８５．７１％，方法回收率９９．７０％～１１０．７％。
Plasma samples were extracted with methylene chloride. ODS column was used with methanol-phosphate buffer-triethylamine (30: 69.8: 0.2) as the mobile phase. The plasma concentration of rabbit oral tenoxicam tablets was determined by RP-HPLC. The linear range was 0.1-30 μg / ml with the lowest detection 0.1 μg / ml (S / N> 2). The extraction recovery was 85.71%, the recovery rate was 99.70% ~ 110.7%.