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用ＤＭＤｃＤＮＡ８探针从假肥大型肌营养不良（Ｄｕｃｈｅｎｎｅｍｕｓｃｕｌａｒｄｙｓｔｒｏｐｈｙ，ＤＭＤ）基因的ＹＡＣ克隆的ｃｏｓｍｉｄ亚克隆库中筛选到９个阳性ｃｏｓｍｉｄ克隆，Ｓｏｕｔｈｅｒｎ杂交鉴定ｃｏｓｍｉｄＣ０４６１含ＤＭＤ基因的第５１号外显子，将该ｃｏｓｍｉｄ亚克隆于ｐＶＣ１１８中，获得含ＤＭＤ基因第５０和５１号内含子的３．１ｋｂ－ＨｉｎｄⅢ片段的亚克隆，将亚克隆的插入片段分别用Ｓａｕ３ＡⅡ完全和部分酶切克隆于ｐＵＣ１１８中，Ｓａｎｇｅｒ法双链测序测出质粒克隆的序列并重叠所测出的序列，确定该片段的长度为３１７９ｂｐ。与Ｓｐｅｅｒ测出的该段序列（３１５９ｂｐ）比较相差２０ｂｐ，有３３处不同。并发现有重复顺序存在，它们之间可能会发生重组并可能导致第５１号外显子的缺失。
Nine positive cosmid clones were screened from the cosmid subclone of YAC clone of Duchenne muscular dystrophy (DMD) gene by using DMD cDNA8 probe. CosmidC0461 containing exon 51 of DMD gene was identified by Southern blot, The subclone of cosmid was subcloned into pVC118 to obtain a subclone of the 3.1kb-HindIII fragment containing the DMD gene No. 50 and 51 introns. The subcloned insert was completely and partially digested with Sau3AII in pUC118. Sanger The double-stranded sequencing method detected the sequence of the plasmid clone and overlaid the measured sequence, and confirmed that the fragment was 3179 bp in length. This sequence differs from Speer’s (3159 bp) by 20 bp in 33 different positions. And found to have a repetitive sequence exists, recombination between them and may lead to the deletion of exon 51.