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目的：对乳腺导管原位癌进行病理形态分析，并行ｃ－ｅｒｂＢ－２、ｐ５３癌基因蛋白、增殖细胞核抗原（ＰＣＮＡ）表达以及相关性的研究，以期为临床判断潜在恶性程度及预后提供参考指标。方法：运用病理形态分析以及枸橼酸－微波－ＡＢＣ免疫组化法对２５例常规福尔马林固定、石蜡包埋乳腺导管原位癌组织进行回顾性研究。结果：（１）２５例乳腺原位导管癌ｃ－ｅｒｂＢ－２、ｐ５３、ＰＣＮＡ表达的阳性率分别为３６．０％，４０．０％和４０．０％；（２）粉刺型ｃ－ｅｒｂＢ－２、ｐ５３、ＰＣＮＡ表达的阳性率均高于非粉刺型，而且ｃ－ｅｒｂＢ－２阳性率相差有显著性（Ｐ＜０．０５）；（３）坏死、核异型性、核分裂数与ｃ－ｅｒｂＢ－２、ｐ５３、ＰＣＮＡ的表达有关，其中，坏死与ＰＣＮＡ阳性表达显著相关（Ｐ＜０．０５），核异型性与ｃ－ｅｒｂＢ－２蛋白表达显著相关（Ｐ＜０．０５）。结论：乳腺导管原位癌无论病理形态还是生物学行为都是异质性的，除了组织学亚型，某些形态指标以及ｃ－ｅｒｂＢ－２癌基因蛋白的表达也可作为恶性度指标。
OBJECTIVE: To analyze the pathological features of ductal carcinoma in situ in situ, and to study the expression of c-erbB-2, p53 oncogene protein, proliferating cell nuclear antigen (PCNA) and their correlation in order to provide a reference for the clinical judgment of potential malignancy and prognosis. . METHODS: Twenty-five routine formalin-fixed, paraffin-embedded breast ductal carcinoma in situ were retrospectively studied by pathological analysis and citrate-microwave-ABC immunohistochemistry. Results: (1) The positive rates of c-erbB-2, p53 and PCNA expression in 25 cases of breast ductal carcinoma in situ were 36.0%, 40.0% and 40.0%, respectively; (2) Acne c-erbB The positive rate of p53 and PCNA expression was higher than that of non-comedo type, and the difference of c-erbB-2 positive rate was significant (P<0.05); (3) Necrosis, nuclear atypia, number of mitosis, and c The expression of -erbB-2, p53 and PCNA was related, among which, necrosis was significantly associated with the positive expression of PCNA (P<0.05), and nuclear atypia was significantly associated with the expression of c-erbB-2 protein (P<0.05). CONCLUSIONS: Ductal ductal carcinoma in situ is heterogeneous regardless of pathological morphology or biological behavior. In addition to histological subtypes, certain morphological markers and c-erbB-2 oncogene protein expression can be used as indicators of malignancy.