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目的探讨新生儿缺氧缺血性脑病血清神经元特异性烯醇酶（ＮＳＥ）与血浆内皮素（ＥＴ）变化及高压氧治疗的作用。方法高压氧治疗以纯氧加压，压力０．０５～０．０７ ＭＰ加压 ２０ ｍｉｎ．稳压 ２０ ｍｉｎ，减压 ２０ ｍｉｎ，共历时 １ｈ，每天一次，疗程５～１０ ｄ。ＮＳＥ活性测定采用酶联免疫分析法；ＥＴ活性测定采用放射免疫分析法。结果中度ＨＩＥ患儿血清ＮＳＥ与血浆ＥＴ浓度分别为（１４．７２±４ ２６）μｇ／Ｌ（７６．１±１９．２）ｎｇ／Ｌ；重度ＨＩＥ患儿血清ＮＳＥ与血浆ＥＴ浓度分别为（１５．６４±５．８２）μｇ／Ｌ，（８２．５±２１．６）ｎｇ／Ｌ；中、重度ＨＩＥ患儿血清ＮＳＥ与血浆ＥＴ浓度显著高于对照组（分别为１２．４７± ３．４９ μｇ／Ｌ， ５６．３２± １６．７ｎｇ／Ｌ）。轻度 ＨＩＥ患儿血清 ＮＳＥ（ １３．５８± ４．５７） μｇ／Ｌ，血浆ＥＴ（６２．４± １８．５） ｎｇ／Ｌ与对照组比较无显著差异。中、重度 ＨＩＥ患儿高压氧治疗后，血清 ＮＳＥ，血浆 ＥＴ降低幅度分别为（ １．９２± ０．４６）μｇ／Ｌ，（ １２．７２± ４． ３７）ｎｇ／Ｌ，明显大于常规治疗组（ Ｐ＜ ０．０５）。结论血清 ＮＳＥ和血浆 ＥＴ是ＨＩＥ早期诊断与疗效判
Objective To investigate the changes of serum neuron-specific enolase (NSE) and plasma endothelin (ET) in neonates with hypoxic-ischemic encephalopathy and the effects of hyperbaric oxygen therapy. Methods hyperbaric oxygen therapy with pure oxygen pressure, pressure 0.05 ~ 0.07 MP pressure 20 min. Regulated 20 min, decompression 20 min, a total of 1h, once daily, course of treatment 5 ~ 10d. NSE activity was measured by enzyme-linked immunosorbent assay; ET activity was measured by radioimmunoassay. Results Serum concentrations of NSE and plasma ET were (14.72 ± 426) μg / L (76.1 ± 19.2) ng / L, respectively. Serum NSE and plasma ET levels in children with severe HIE were (15.64 ± 5.82) μg / L and (82.5 ± 21.6) ng / L, respectively. Serum levels of NSE and plasma ET in children with moderate and severe HIE were significantly higher than those in the control group (12.47 ± 3.49 μg / L, 56.32 ± 16.7 ng / L). There was no significant difference in serum NSE (13.58 ± 4.57) μg / L and plasma ET (62.4 ± 18.5) ng / L between mild HIE and control group. After hyperbaric oxygen therapy, the reduction of serum NSE and plasma ET in moderate and severe HIE children were (1.92 ± 0.46) μg / L and (12.72 ± 4.37) ng / L, respectively, which were significantly higher than that of routine treatment Group (P <0.05). Conclusion Serum NSE and plasma ET are the early diagnosis and treatment of HIE