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目的阐明在癫发作与发展中起着重要作用的脑内兴奋性氨基酸类递质和内阿片肽之间的关系。方法应用原位杂交方法观察了青霉素致及海马内微量注射ＮＭＤＡ受体阻断剂ＭＫ－８０１（５－ｍｅｔｈｙ１－１０，１１－ｄｉｈｙｄｒｏ－５Ｈ－ｄｉｂｅｎｚｏ［ａ，ｄ］ｃｙｃｌｏｈｅｐｔａｎ－５，１０－ｉｍｉｎｅｍａｌｅａｔｅ）和非ＮＭＤＡ受体阻断剂ＤＮＱＸ（６，７－ｄｉｎｉｔｒｏｑｕｉｎｏｘａｌｉｎｅ－２，３－ｄｉｏｎｅ）后，大鼠海马内前脑啡肽原和前强啡肽原ｍＲＮＡ表达的变化。结果在青霉素致４ｈ后，海马ＣＡ１区、ＣＡ３区、齿状回前脑啡肽原ｍＲＮＡ和海马ＣＡ３区、齿状回前强啡肽原ｍＲＮＡ的表达明显增加。在青霉素致前分别在海马内微量注射ＭＫ８０１（６μｇ）和ＤＮＱＸ（４μｇ），则在减弱发作的同时，海马内前脑啡肽原ｍＲＮＡ的表达明显减少，而前强啡肽原ｍＲＮＡ的表达继续增加。结论青霉素致引起大鼠脑内脑啡肽和强啡肽的变化可能和ＮＭＤＡ受体及非ＮＭＤＡ受体对其基因表达的调节有关
Objective To elucidate the relationship between intracerebral excitatory amino acid neurotransmitters and endo-opioid peptides, which play an important role in the onset and development of epilepsy. Methods In situ hybridization was used to observe the effects of MK-801 (5-methy1-10,11-dihydro-5H-dibenzo [a, d] cycloheptan- The changes of proenkephalin and preproenorphin mRNA in hippocampus of rats after imine maleate and non-NMDA receptor antagonist DNQX (6,7-dinitroquinoxaline-2,3-dione). Results After 4 hours of penicillin, the expression of preproenkephalin mRNA in hippocampal CA1, CA3, dentate gyrus and hippocampal CA3 region and dentate gyrus increased significantly. Microinjection of MK801 (6 μg) and DNQX (4 μg) into the hippocampus prior to penicillin-induced encephalitis, respectively, attenuated seizures and significantly reduced the expression of proenkephalin mRNA in the hippocampus. However, The expression continues to increase. Conclusion The changes of enkephalin and dynorphin in brain induced by penicillin may be related to the regulation of gene expression by NMDA receptors and non-NMDA receptors