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为解析HMGR基因在杜仲橡胶生物合成中的作用,本研究以杜仲良种华仲6号叶片和果皮为材料,参考杜仲基因组和转录组数据,克隆了杜仲HMGR基因,命名为Eu HMGR,全长1770 bp,编码590个氨基酸。实时荧光定量PCR测定结果显示,EuHMGR基因在果皮中的表达量于5月中旬达到最大值,而在叶片中的最大值出现在7月中旬。通过索氏提取测定相应时期果皮和叶片的含胶量表明,果皮中的含胶量从4月中旬到5月下旬迅速增加,而叶片中的含胶量一直平稳增长。分析不同发育时期果皮和叶片Eu HMGR基因表达量和含胶增长速率相关性发现,果皮Eu HMGR基因在不同发育时期的相对表达量和含胶增长速率呈显著正相关,而二者在叶片中无明显相关性。因此,推测Eu HMGR基因的表达与杜仲果皮中橡胶生物合成相关。
In order to elucidate the role of HMGR gene in the biosynthesis of Eucommia ulmoides rubber, the Eucommia ulmoides HMGR gene was cloned from Eucommia ulmoides oak leaves and pericarp with reference to the Eucommia genome and transcriptome data. bp, encoding 590 amino acids. Real-time PCR results showed that the expression level of EuHMGR gene in the pericarp reaches the maximum in mid-May, while the maximum value in the leaf appears in mid-July. Soxhlet extraction determined by the corresponding period of the pericarp and leaf gum content shows that the pericarp gel content from mid-April to late May increased rapidly, while the leaf gum content has been steadily growing. The correlation between the expression level of Eu HMGR gene and the rate of gel growth at different developmental stages was analyzed. It was found that the relative expression level of Eu HMGR gene at different developmental stages was significantly and positively correlated with the growth rate of the gel. However, Clearly relevant. Therefore, it is speculated that the expression of Eu HMGR gene is related to the rubber biosynthesis in the eucommia bark.