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应用代表性差异分析 (cDNARDA)技术 ,对类似普通 2型糖尿病大鼠肾脏组织基因差异表达进行筛查 ,初步探讨类似普通 2型糖尿病大鼠肾脏损害发病的分子机制 .首先以类似普通 2型糖尿病大鼠肾脏组织作为实验组 (Tester) ,正常大鼠肾脏作为对照组或驱动组 (Driver)通过cDNARDA进行基因差异表达筛查 ;最终的差异产物亚克隆到Puc 18载体 ,测序及并进行生物信息学分析 ;半定量RT PCR对筛查到新的基因进行初步的鉴定 .结果发现 9个新ESTs ,2个新基因 .这 2个新基因分别与人及小鼠的丝氨酸蛋白酶抑制因子F ,及真核细胞转录启动因子 3亚单位 5 (EIF 3epsilon)基因有高度的相似性 (>90 % )并在类似普通 2型糖尿病大鼠肾脏组织表达上调 .推测 2个新基因分别是大鼠的丝氨酸蛋白酶抑制因子F及真核细胞转录启动因子 3亚单位 5 .两个新基因在类似普通 2型糖尿病大鼠肾脏组织表达上调 ,可能与类似普通 2型糖尿病大鼠肾脏损害相关 .同时 ,对新基因RS91进行了全长cDNA克隆
The representative differential expression analysis (cDNARDA) technique was used to screen the differentially expressed genes in kidney tissue of common type 2 diabetic rats to explore the molecular mechanism of kidney damage in common type 2 diabetic rats.Firstly, with common type 2 diabetes mellitus The rat kidney tissue was used as the experimental group (Tester), and the normal rat kidney was used as a control or driver for gene differential expression screening by cDNARDA. The final differential product was subcloned into the Puc 18 vector, sequenced and bioinformatics The results showed that there were 9 new ESTs and 2 new genes.These two new genes were respectively associated with human and mouse serine protease inhibitor F and EIF 3epsilon gene was highly similar (> 90%) and upregulated in the kidney tissue of common type 2 diabetic rats.It is speculated that the two new genes are serine Protease inhibitor F and eukaryotic transcription factor 3 subunit 5. Two new genes in normal type 2 diabetic rats kidney tissue upregulation, Which may be related to the kidney damage of common type 2 diabetic rats.At the same time, a full-length cDNA clone of the new gene RS91