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目的:芒果苷、槲皮素和没食子酸都是芒果叶的主要成分,含量均超过2%。本研究探讨芒果苷分别与槲皮素、没食子酸合用时,在镇咳作用上是否存在交互影响。方法:小鼠40只,雌雄各半,随机分为生理盐水组,生理盐水+芒果苷组(0.1 g.kg-1),生理盐水+槲皮素组(0.1 g.kg-1),生理盐水+芒果苷+槲皮素组(0.1+0.1 g.kg-1),每组10只,采用浓氨水致咳的小鼠模型研究镇咳作用,记录小鼠3 min内的咳嗽次数。采用析因方差分析判断药物间是否存在交互影响。结果:与生理盐水组比较,芒果苷组有镇咳作用(P<0.05),槲皮素组和没食子酸组则无(P>0.05)。芒果苷+槲皮素组的镇咳作用明显强于芒果苷组(P<0.01);芒果苷+没食子酸组的镇咳作用与芒果苷组相当(P>0.05)。结论:芒果苷和槲皮素合用,有协同镇咳作用,能明显提高芒果苷的镇咳药效。芒果苷与没食子酸之间没有交互影响,两者合用,只表现出芒果苷的镇咳药效。
Objective: Mangiferin, quercetin and gallic acid are the main components of mango leaves, the content of more than 2%. This study was to explore whether mangiferin interacts with quercetin and gallic acid in the antitussive effect. Methods: Forty mice were randomly divided into three groups: normal saline group, saline + mangiferin group (0.1 g.kg-1), saline + quercetin group (0.1 g.kg-1) Saline + mangiferin + quercetin group (0.1 + 0.1 g.kg-1), with 10 mice in each group. The antitussive effect was studied in a mouse model of hyperoxia-induced cough, and the number of coughs within 3 min was recorded. Factorial analysis of variance (ANOVA) was used to determine if there was any interaction between drugs. Results: Compared with the saline group, the anti-cough effect was observed in the mangiferin group (P <0.05), but not in the quercetin and gallic acid groups (P> 0.05). The antitussive effect of mangiferin + quercetin group was stronger than that of mangiferin group (P <0.01). The antitussive effect of mangiferin + gallic acid group was similar to that of mangiferin group (P> 0.05). Conclusion: Mangiferin combined with quercetin, synergistic antitussive effect, can significantly improve the efficacy of anti-cough glycosides. Mangiferin and gallic acid did not interact with each other, the two combined, showing only the anti-cough efficacy of mangiferin.