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目的：以核糖体转录间隔区(rDNA ITS)序列为分子标记，对1种产自贵州安顺的华钩藤植物进行遗传分析与分子鉴定。方法：通过改良CTAB法提取样品总DNA，利用通用引物对rDNA ITS序列进行PCR扩增，经克隆、测序后，运用Clustal X、BioEdit和PAUP等软件进行序列分析和构建系统发育树。结果：测序得到样品的rDNA ITS区序列长度为719bp，序列分析结果显示其与Genbank中已有的华钩藤[Uncaria sinensi(Oliv.)Havil.] rDNA ITS区序列之间相似性达99.8%，并且在系统发育树中并排聚类成1支。结论：基于rDNA ITS区序列的测序分析和系统发育树构建的分子生物学方法，能够对华钩藤植物进行准确的分子鉴定，为钩藤属中药材的种类鉴定和种间分类提供分子生物学理论依据。“,”Objective:To characterize the phylogenetic relationship of one Guizhou Uncaria sinensis(Oliv.)Havil,molecular identification and genetic analysis were carried out using the rDNA ITS sequence as molecular marker. Methods:Molecular identification and genetic analysis were carried out with the rDNA ITS sequence as molecular marker.Total DNA was extracted with modified CTAB Method:and there by rDNA ITS regions were amplified with primers ITS4/ITS5, sequenced and phylogenetic analysed with Clustal X, BioEdit and PAUP. Results:The entire rDNA ITS sequence of Uncaria was 719bp.Sequence analysis showed that the rDNA ITS sequence was closely related to Uncaria sinensis(Oliv. ) Havil available in GenBank and the similarity reached 99.8%. Conclusion Based on molecular biology Methods:of rDNA ITS region analysis, molecular identification is useful inaccurate classification on Uncaria sinensis(Oliv.)Havil.,which provides molecular evidence of taxonomy and identification of different species in genus Uncaria.