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目的:探讨用固相萃取法提取人参再造丸中总生物碱,并用 HPLC 测定小檗碱含量的方法.方法:用固相萃取小柱提取人参再造丸中的总生物碱,用HPLC法测定其中小檗碱的含量,色谱条件:以乙腈∶0.05mol/l KH2PO4缓冲液=28:72(V:V)为流动相,流速1.0ml/min;选用YMC-pack ODS-A (150×4.6mm,5μm)色谱柱;柱温40℃;紫外检测波长345nm;进样量10μl.结果:小檗碱的提取回收率为85.0%,回收率为101.0,HPLC 测定方法在浓度5~50μg/mL 范围内线性良好(r =0.9992),批内和批间 RSD=0.5%,1.0%.结论:本法样品处理简便、快速,杂质干扰小,测定结果准确可靠,也可用于人参再造丸中其他生物碱的定量分析.“,”[ABSTRACT] OBJECTIVE: To improve determination methods of Berberines in Renshenzaizaowan. METHOD: Berberine and other Alkaloidin in Renshenzaizaowan were extracted by solid phase extract column from its diluted solution of methanol extraction and its content was determined by HPLC. The YMC-pack ODS-A C18 was used as separation column (150×4.6mm, 5μm) and the substance was eluted by mobile phase: V:V=acetonitrile:0.05mol/l KH2PO4=28:72, the flow rate was 1.0ml/min. DAD detector was used and detecting wave length was 345nm; the column temperature was 40℃ . RESULT: The linear range was 5 ~ 50 μg ( r = 9999). The average recovery of Berberine was 101.0 %. CONCLUSION: The method was rapid and simple, the peak disturbs slightly. The result was accurately and the method also applicable for quantitative analysis of other Alkaloid in Renshenzaizaowan.