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目的 :了解P16基因在膀胱移行细胞癌 (TCC)中甲基化改变及这一改变与肿瘤恶性程度的关系。方法 :采用PCR法检测 31例膀胱TCCs标本及 9例正常膀胱粘膜P16基因甲基化。结果 :正常膀胱粘膜无甲基化P16基因外元 1甲基化率为 2 9.0 3% ,外元 2为 35 .48% ;外元 1甲基化与肿瘤恶性程度有显著相关性 (P <0 .0 1) ,外元 2甲基化与肿瘤恶性程度无相关性 (P >0 .0 5 )。结论 :膀胱TCCP16基因存在甲基化 ,外元 1甲基化可使细胞增殖进一步失控而使肿瘤恶性程度增加 ,在P16基因失活中可能起更重要作用。
Objective: To investigate the methylation changes of P16 gene in transitional cell carcinoma of the bladder (TCC) and its relationship with the degree of tumor malignancy. Methods: Thirty-one cases of bladder TCCs and nine cases of normal bladder mucosa were methylated by PCR. Results: The methylation rate of methylation-free P16 gene in normal bladder mucosa was 29.03% and 35.48% respectively. The methylation of exon 1 was significantly associated with the degree of malignancy (P < There was no correlation between the methylation of exon 2 and the degree of malignancy (P> 0.05). CONCLUSION: Methylation of bladder TCCP16 gene exists in the bladder. Methylation of the extracellular 1 methylation may further control the cell proliferation and increase the degree of malignancy. It may play a more important role in the inactivation of P16 gene.