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目的 内脏高敏感性是肠易激综合征 (IBS)的重要病理生理特征之一 ,该文旨在建立内脏高敏性动物模型 ,并用两种方法验证该模型的有效性。方法 取出生后 8～ 2 1d的大鼠 ,每天给予直肠内醋酸刺激 ,分别在出生后 6、8及 10周 ,对这些成年后的大鼠进行直肠扩张 ,评估其腹部收缩反射(AWR)阈值 ;并在出生 12周后测定大鼠腹壁肌电活动 ,验证敏感性有无异常改变。结果 与新生期生理盐水刺激组 (NS组 )和成年醋酸刺激组 (AA组 )相比 ,直肠扩张时 ,新生期醋酸刺激组大鼠 (NA组 )腹部抬高和背部拱起的容量阈值显著降低 (P值均 <0 .0 1) ;0 .5、0 .8、1.2ml扩张容量下腹壁肌电活动明显增强 (P分别 <0 .0 1,<0 .0 5和 0 .0 5 )。结论 新生期肠道内的慢性刺激 ,可以在成年后引起慢性内脏敏感性增高 ,而肠黏膜未见异常病理改变 ,符合IBS的基本特征。
Objective The visceral hypersensitivity is one of the important pathophysiological features of irritable bowel syndrome (IBS). This paper aims to establish a visceral hypersensitivity animal model and verify the validity of the model by two methods. METHODS: Rats were sacrificed 8 to 21 days after birth and were intrarectally administered with acetic acid. These adult rats were rectally dilated at 6, 8 and 10 weeks after birth to evaluate their abdominal contractive reflex (AWR) threshold ; And after 12 weeks of life measured myoelectrical activity in the abdominal wall of rats to verify the sensitivity of any abnormal changes. Results Compared with neonatal NS group and AA group, the thresholds of abdomen elevation and dorsal arching in neonatal acetate-stimulated rats (NA group) were significantly increased when rectum was dilated (P <0.01); 0.05, 0.08, 1.2ml expansion capacity of the abdominal wall EMG activity was significantly enhanced (P <0.01, <0.05 and 0.55 ). Conclusions Chronic stimulation in the newborn intestine may cause chronic visceral hypersensitivity in adult, but no abnormal pathological changes in intestinal mucosa, which is consistent with the basic characteristics of IBS.