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[Objective]To study the effect of curcumin on the proteome of HepG2 cells by using two-dimensional electrophoresis and mass spectrum.[Methods] The proteins from HepG2 cells and HepG2 cells treated with 30 μmol/L curcumin were separated with two-dimensional electrophoresis and the gels were scanned after silver staining.All images were analyzed by Image Master 6.0 software,and the different pro-teins were identified by mass spectrum.[Result] The expression of protein disulfide isomerase,replication protein A2,carnitine deficiency-associated gene and cyclin-dependent kinase inhibitor 2A increased in the HepG2 cell treated with 30 μmol/L curcumin,while the expression of PCNA,ER-60 protease,Ran binding protein 1,Stathmin 1/oncoprotein 18,Nm23 protein decreased.[Conclusion] Curcumin could in-hibit tumor cell growth,interfere cell cycle,promote apoptosis,inhibit tumor invasion and metastasis,and promote cell repair.In addition,the hypolipidemic and anti-atherosclerosis effects of curcumin may correlate with promotion of the transmembrane transport of fatty acids and maintain of cell membrane stability.
[Objective] To study the effect of curcumin on the proteome of HepG2 cells by using two-dimensional electrophoresis and mass spectra. [Methods] The proteins from HepG2 cells and HepG2 cells treated with 30 μmol / L curcumin were separated with two-dimensional electrophoresis and the gels were scanned after silver staining. All images were analyzed by Image Master 6.0 software, and the different pro-teins were identified by mass spectrum. [Result] The expression of protein disulfide isomerase, replication protein A2, carnitine deficiency-associated gene and cyclin-dependent kinase inhibitor 2A increased in the HepG2 cell treated with 30 μmol / L curcumin, while the expression of PCNA, ER-60 protease, Ran binding protein 1, Stathmin 1 / oncoprotein 18, Nm23 protein decreased. could in-hibit tumor cell growth, interfere cell cycle, promote apoptosis, inhibit tumor invasion and metastasis, and promote cell repair. In addition, the hypolipidemic and anti-atherosclerosis effects of curcumin may correlate with promotion of the transmembrane transport of fatty acids and maintain of cell membrane stability.