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为了解AGM3基因在异源植物中对开花及花器官发育的影响,采用农杆菌介导法将dominant negative mutation(DNM)结构基因35S-AGM3-E9导入烟草(Nicotianatabacum),经PCR和Southern检测获得了一批阳性转化植株。荧光定量分析结果显示,AGM3在各个转基因株系中均有表达,且不同株系间表达量差异极显著。调查结果分析表明:与野生型植株相比,46.7%的转基因株系未成花,33.3%的转基因株系开花时间平均推迟29.7天,20%的转基因株系开花时期与野生型相同。对花器官形态的观察发现,转基因烟草出现花萼数目减少、花冠产生深裂、花瓣和雄蕊形状改变、雄蕊数目增多或减少等变异。这些研究结果表明35S-AGM3-E9有效地抑制了花的发育,为利用转基因手段获得不育材料的研究奠定了基础。
To understand the effect of AGM3 gene on flowering and floral organ development in heterologous plants, the dominant negative mutation (DNM) structural gene 35S-AGM3-E9 was introduced into Nicotiana tabacum by Agrobacterium-mediated transformation. A number of positive transformed plants. Fluorescence quantitative analysis showed that AGM3 was expressed in all the transgenic lines, and the expression difference between different lines was extremely significant. The results of the survey showed that 46.7% of the transgenic lines did not flower compared with the wild type plants, 33.3% of the transgenic lines delayed the average of 29.7 days of flowering time, and 20% of the transgenic lines had the same flowering stage as the wild type. Morphological observation of floral organs revealed that the number of calyxes in transgenic tobacco decreased, the corolla had deep cleavage, the shape of petals and stamens changed, and the number of stamens increased or decreased. These results indicated that 35S-AGM3-E9 effectively inhibited the flower development and laid the foundation for the study of obtaining sterile material by transgenic means.