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目的:观察Smurf1、Smurf2、Smad3和Smad蛋白在人肝纤维化组织中的表达,探讨四者的相互关系及介导的信号传导在肝纤维化发生中的作用机制.方法:采用免疫组织化学法测定9例正常肝组织和38例慢性乙型肝炎病毒(hepatitis B virus,H B V)感染者肝组织中S m u r f1、S m u r f2、Smad3和Smad7的表达情况.结果:Smurf1、Smurf2、Smad3和Smad7在肝内实质细胞及非实质细胞均可见广泛表达.与正常肝相比,肝纤维化组Smad3、Smurf2的阳性表达率显著增加(66.7%vs 100%、66.7%vs 92.1%,P<0.01、P<0.01),Smad7阳性表达率显著降低(77.8%vs 39.5%,P<0.01);而Smurf1阳性表达率无显著变化(77.8%vs 63.2%,P>0.05),差异无统计学意义.Smad3、Smurf2与纤维化程度呈显著正相关(P<0.01、P<0.01);S m a d7与纤维化程度呈显著负相关(P<0.01);Smurf1与肝纤维化无显著相关性(P>0.05).Smurf2与Smad3呈显著正相关(P<0.01),与S m a d7呈显著负相关(P<0.01);Smurf1与Smurf2、Smad3、Smad7均无相关性(均P>0.05);Smad3与Smad7呈负相关(P<0.01).结论:Smad3信号增强及Smad7信号缺失可能导致肝纤维化发展,Smurf2在肝纤维化进展中可能发挥双向调节作用.
OBJECTIVE: To observe the expression of Smurf1, Smurf2, Smad3 and Smad in human hepatic fibrosis tissue and to explore their correlation and the mechanism of signal transduction in the pathogenesis of hepatic fibrosis.Methods: Immunohistochemistry The expression of S mur f1, S mur f2, Smad3 and Smad7 in liver tissues of 9 normal liver tissues and 38 patients with hepatitis B virus (HBV) infection were determined.Results: The expressions of Smurf1, Smurf2, Smad3 and Smad7 The expression of Smad3 and Smurf2 in liver fibrosis group was significantly higher than that in normal liver (66.7% vs 100%, 66.7% vs 92.1%, P <0.01, (77.8% vs 63.2%, P> 0.05), and there was no significant difference between the two groups (P <0.01, P <0.01), the positive expression rate of Smad7 was significantly lower (77.8% vs 39.5%, P <0.01) There was a significant positive correlation between Smurf2 and fibrosis (P <0.01, P <0.01); S ma d7 was significantly negatively correlated with fibrosis (P <0.01); Smurf1 had no significant correlation with fibrosis ). Smurf2 was positively correlated with Smad3 (P <0.01), negatively correlated with S ma d7 (P <0.01). Smurf1 and Smurf2 Smad3 and Smad7 (all P> 0.05), Smad3 was negatively correlated with Smad7 (P <0.01) .Conclusion: Smad3 and Smad7 deficiency may lead to the development of hepatic fibrosis, Smurf2 may play an important role in the progress of hepatic fibrosis Play a two-way regulatory role.