【摘 要】
:
Soil samples were collected from three plots under different land utilization patterns including degradation,farming,and restoration.The abundances of methanotr
【机 构】
:
State Key Laboratory of Urban and Regional Ecology,Key Laboratory of Systematic Mycology and Licheno
【基金项目】
:
the Knowledge Innovation Program of Chinese Academy of Sciences;the Natural Science Foundation of China
论文部分内容阅读
Soil samples were collected from three plots under different land utilization patterns including degradation,farming,and restoration.The abundances of methanotrophs were quantified using real-time polymerase chain reaction(PCR)based on the pmoA and 16S rRNA genes,and the community fingerprint was analyzed using denaturing gradient gel electrophoresis(DGGE)aiming at pmoA gene.Significantly lower 16S rRNA and pmoA genes copies were found in the degradation treatment than in farming and restoration.Higher abundances of Type Ⅰ than those of Type Ⅱ methanotrophs were detected in all treatments.The treatment of farming was clearly separated from degradation and restoration according to the DGGE profile by cluster analysis.The lowest diversity indices were observed in the F(farming plot),suggesting that the community structure was strongly affected by farming activities.There were significantly positive correlations between the copy numbers of pmoA also Type Ⅱ-related 16S rRNA genes and soil available K content.Strong negative and positive correlations were found between Type Ⅰ and soil pH,and available P content,respectively.We concluded that the vegetation cover or not,soil characteristics including pH and nutrients of P and K as a result of anthropogenic disturbance may be key factors affecting methanotrophic communities in upland soil.
其他文献
GPR has become an important geophysical method in UXO and landmine detection, for it can detect both metal and non-metallic targets. However, it is difficult to
Aiming at localizing the telemetric capsule for detecting gastrointestinal physiological parameters in vivo accurately, a portable alternating current ( AC ) el
Paulownia fortunei has been successfully used in the phytoremediation of many Pb/Zn mine tailings.However,seed germination and young seedlings of P fortunei rar
The free enzyme extracted from WZ-I,which was identified as Fusarium LK.ex Fx,could effectively degrade chlorpyrifos,an organophosphate insecticide.The methods
Tannery wastewater causes serious ecological and sanitary damage.Chemical analysis of water from Binlamdoune River of the medina of Fez was conducted and the re
To solve the fault diagnosis problem of liquid propellant rocket engine ground testing bed, a fault diagnosis approach based on self-organizing map (SOM) is pro
Background Endoscopic treatment for urethral stricture, including cold knife and laser, poses a major challenge to clinical practice. Both the benefits and draw
The relationship between cloud amount and sea surface temperature (SST) over Western Tropical Pacific cloudy regions during TOGA COARE is investigated based on
Angiotensinogen is a member of the serpin family. It is produced constitutively and released into the circulation mainly by the liver. Angiotensinogen forms ang
Tracing the sediment delivery from its source terrain to its ultimate sink envisage multiple factors that play a vital role in understanding present day erosion