论文部分内容阅读
目的建立UPLC法测定赤防通络止痛胶囊中芍药苷含量的方法。方法采用超高效液相色谱法,色谱柱为BEH C18(2.1 mm×50 mm,1.7 um);流动相为乙腈-0.1%磷酸(14:86);流速:0.5 mL/min,检测波长:230 nm;柱温26℃。结果芍药苷进样量在0.0113~0.2260 ug范围内与峰面积成良好的线性关系,R=0.9996。平均加样回收率为97.37%,RSD为2.07%。结论该测定方法简单、准确、重复性好,可用于赤防通络止痛胶囊中芍药苷含量的测定。
Objective To establish a method for the determination of paeoniflorin in Chifeng Tonglu Zhitong Capsules by UPLC. Methods The mobile phase was BEH C18 (2.1 mm × 50 mm, 1.7 μm) with acetonitrile-0.1% phosphoric acid (14:86) at a flow rate of 0.5 mL / min and the detection wavelength was 230 nm; column temperature 26 ℃. Results Paeoniflorin injection volume in the range of 0.0113 ~ 0.2260 ug with the peak area into a good linear relationship, R = 0.9996. The average recovery was 97.37% with a RSD of 2.07%. Conclusion The method is simple, accurate and reproducible. It can be used for the determination of paeoniflorin in Chifeng Tonglu Zhitong Capsule.