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为探索稀土化合物在防治前列腺癌骨转移方面的潜在药理作用,研究了镧(La)和钆(Gd)对前列腺癌细胞DU145诱导的成、破骨细胞分化的影响.实验结果表明,DU145细胞的条件培养基能够促进小鼠前成骨细胞MC3T3-e1的增殖、ALP活性和钙沉积,而且经La处理后作用进一步加强.然而Gd处理后的DU145细胞条件培养基组对MC3T3-e1的ALP活性和钙沉积影响并不显著.RT-PCR实验表明,La可能通过上调DU145细胞分泌的细胞因子BMP6表达,抑制Noggin的表达进而促进成骨细胞的增殖和分化;而且成骨细胞的增殖、分化与JNK信号转导通路有关.此外,还观察到La能够抑制DU145细胞诱导的破骨细胞生成,并且通过下调前列腺癌细胞RANKL的表达而起到抑制作用.然而Gd对DU145细胞诱导的破骨细胞生成的影响并不显著.
In order to explore the potential pharmacological effects of rare earth compounds on the prevention and treatment of prostate cancer bone metastasis, the effects of lanthanum (La) and gadolinium (Gd) on the differentiation of osteoclasts and osteoblasts induced by DU145 were studied.The experimental results showed that DU145 cells Conditioned medium promoted the proliferation, ALP activity and calcium deposition of pre-mouse osteoblast MC3T3-e1, and further enhanced the effect of La treatment. However, the ALP activity of MC3T3-e1 by Gd-treated DU145 cell conditioned medium group And calcium deposition was not significant.RT-PCR experiments showed that La may increase osteoblast proliferation and differentiation by up-regulating the expression of BMP6 secreted by DU145 cells, inhibiting the expression of Noggin, JNK signal transduction pathway.In addition, La was also observed to inhibit DU145 cell-induced osteoclastogenesis, and by downregulating the expression of prostate cancer cells RANKL.However, Gd on DU145 cells induced osteoclastogenesis The impact is not significant.