论文部分内容阅读
目的了解临床分离的耐药性鲍曼不动杆菌携带抗药基因情况及其对常用消毒剂抗性水平。方法采用基因扩增法和悬液定量杀菌试验方法,对临床分离的耐药性鲍曼不动杆菌qacE△Ⅰ-sul Ⅰ基因携带率和对3种常用消毒剂抗性变化情况进行评价。结果临床分离的10株耐药性鲍曼不动杆菌中有9株qacE△Ⅰ-sul Ⅰ基因阳性。用浓度为250 mg/L有效氯或有效碘的消毒液,作用1 min,对悬液内临床分离鲍曼不动杆菌平均杀灭率均达到99.9%以上;用浓度为250 mg/L的戊二醛消毒液作用3 min,对临床分离的鲍曼不动杆菌杀灭率为100%。结论临床分离耐药鲍曼不动杆菌抗药基因携带率较高,但常规浓度的消毒剂均可有效杀灭,提示其对消毒剂抗性无明显变化。
Objective To understand the clinical drug resistance of Acinetobacter baumannii carrying drug-resistant gene and its resistance to commonly used disinfectants. Methods The genetic susceptibility to Acinetobacter baumannii (qBEE Ⅰ-sul Ⅰ) and its resistance to three common disinfectants were evaluated by gene amplification and suspension quantitative germicidal test. Results Nine strains of Acinetobacter baumannii isolated clinically were positive for qacE △ Ⅰ-sul Ⅰ gene. The average killing rate of Acinetobacter baumannii in the suspension was over 99.9% with a disinfectant solution of available chlorine or available iodine at a concentration of 250 mg / L for 1 min. Dialdehyde disinfectant for 3 min, the killing rate of clinically isolated Acinetobacter baumannii was 100%. Conclusion The clinical isolates of drug-resistant Acinetobacter baumannii have a higher carrying rate of drug-resistant genes, but conventional disinfectants can effectively kill them, suggesting no significant changes in their resistance to disinfectants.