用CM-Sephadex C-50柱层析,醋酸铵缓冲液洗脱,从平颏海蛇毒腺提取物中分得15个蛋白组分。其中2个有磷脂酶A_2活性的用免疫学的方法证实的肌肉毒组分。10个能作用突触后膜的神经毒组分。肌肉毒的蛋白量占粗毒总蛋白量的32.2％。神经毒的蛋白量占粗毒总蛋白量的52.3％。神经毒的毒性比肌肉毒的毒性强。这些结果提示,平颏海蛇毒有肌肉毒和神经毒,神经毒是平颏海蛇毒的主要毒性成分。 Using CM-Sephadex C-50 column chromatography, elution with ammonium acetate buffer, 15 protein fractions were isolated from the extracts of the sea venom. Two of these were immunologically proven muscle toxic components with phospholipase A2 activity. Ten neurotoxic components that act on the postsynaptic membrane. Muscle toxic protein accounts for 32.2% of total crude protein. Neurotoxic protein accounts for 52.3% of the total crude protein. Neurotoxicity is more toxic than muscle toxicity. These results suggest that Holotrichia californica has muscle toxicity and neurotoxicity, and neurotoxicity is the main toxic component of Holotrichia californica.