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一、材料和方法1. 材料:雄性SD大鼠(体重300～330g)及弱瘤脂肪酸(FFA)和ATPase测试盒等。2.方法:(1)采用大鼠动脉腔内插线法局灶性脑缺血模型,在缺血2h后进行再灌注。(2)再灌注2h后留取缺血半球标本,依据FFA和ATPase测试盒提供的方法,制备组织匀浆,并测定FFA水平及Na+ -K+ -ATP
Materials and Methods 1. Materials: male SD rats weighing 300-330 g, FFA and ATPase test kit. Methods: (1) The rat model of focal cerebral ischemia was established by intraluminal intimal hyperplasia, and reperfusion was performed 2h after ischemia. (2) The ischemic hemispheres were collected 2 h after reperfusion, and homogenates were prepared according to the methods provided by FFA and ATPase test kits. The levels of FFA and Na + -K + -ATP