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目的:观察桂枝挥发油及其主要成分桂皮醛体外对甲型流感病毒A/PR/8/34(H1N1)增殖的影响及对该流感病毒株感染小鼠的治疗作用。方法:MTT法检测受试药物对狗肾传代细胞(MDCK)的半数中毒浓度(TC50)及最大无毒浓度(TC0);血凝法测定甲型流感病毒(H1N1)对MDCK细胞的感染性;MTT法和细胞病变法(CPE)测定两种药物体外抑制流感病毒增殖的有效浓度(IC50)和治疗指数(TI)。计算肺指数及HE染色观察药物对流感病毒(H1N1)感染小鼠的治疗作用。结果:桂枝挥发油和桂皮醛对MDCK细胞的TC50分别为5.38×10-3mg/ml和5.49×10-3mg/ml,TC0均为2.5×10-3mg/ml。桂枝挥发油和桂皮醛能抑制流感病毒在MDCK细胞内的增殖,其IC50分别为5.80×10-5mg/ml与5.31×10-5mg/ml,TI分别为92.82和103.35。桂枝挥发油0.174mg/kg和桂皮醛0.132mg/kg连续灌胃5d,明显降低病毒感染小鼠的肺指数,抑制率分别为26.7%和27.4%,并对病理组织形态有改善作用。结论:桂枝挥发油与桂皮醛体外明显抑制甲型流感病毒(H1N1)在MDCK细胞中的增殖,并对流感病毒株感染小鼠有较好的治疗作用。表明桂枝挥发油及桂皮醛具有抗甲型流感病毒作用,桂皮醛是桂枝挥发油抗病毒效应的主要有效成分之一。
OBJECTIVE: To observe the effects of essential oil of caraway seed and its cinnamic aldehyde on the proliferation of influenza virus A / PR / 8/34 (H1N1) in vitro and its therapeutic effect on mice infected with this strain of influenza virus. Methods: MTT assay was used to determine the median lethal concentration (TC50) and the maximum nontoxic concentration (TC0) of MDCK in MDCK cells. The hematocrit assay was used to determine the infectivity of influenza virus type 1 (H1N1) on MDCK cells. MTT assay and cytopathic assay (CPE) were used to determine the effective concentration (IC50) and therapeutic index (TI) of the two drugs to inhibit influenza virus proliferation in vitro. The pulmonary index and HE staining were used to observe the therapeutic effect of the drug on influenza virus (H1N1) -infected mice. Results: The TC50 of volatile oil and cinnamic aldehyde in MDCK cells were 5.38 × 10-3mg / ml and 5.49 × 10-3mg / ml respectively, and TC0 was 2.5 × 10-3mg / ml respectively. The volatile oil of cinnamon and cinnamic aldehyde inhibited the proliferation of influenza virus in MDCK cells with IC50 of 5.80 × 10-5mg / ml and 5.31 × 10-5mg / ml respectively, with TI of 92.82 and 103.35, respectively. Guizhi volatile oil 0.174mg / kg and cinnamic aldehyde 0.132mg / kg gavage for 5 days, significantly reduced the lung index of virus-infected mice, the inhibition rates were 26.7% and 27.4%, respectively, and the pathological morphology improved. Conclusion: Guizhi volatile oil and cinnamic aldehyde significantly inhibit the proliferation of influenza virus type 1 (H1N1) in MDCK cells in vitro and have a good therapeutic effect on mice infected with influenza virus strains. It showed that the essential oil of cassia twig and cinnamic aldehyde had anti-influenza A virus activity, and cinnamaldehyde was one of the main active ingredients of the anti-virus effect of cassia twig volatile oil.