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以籼型细胞质雄性不育系珍汕９７Ａ、保持系珍汕９７Ｂ、恢复系明恢６３和两个Ｆ１杂种为对象分析了线粒体ＤＮＡ间的多态性．用６个线粒体ＤＮＡ探针对５种来源的线粒体ＤＮＡ进行Ｓｏｕｔｈｅｒｎ印迹分析．用ＣｏｘⅡ、ａｔｐＡ、２６ｓｒＲＮＡ为探针进行杂交时，不育系、保持系和恢复系之间具有相同的杂交结果，而用ＣｏｘⅠ、ＣｏｘⅡ、ａｔｐ６和６３０ｂｐｍｔＤＮＡ片段为探针杂交时，不育系和可育系之间出现了不同的杂交带，表明不育系和可育系的ｍｔＤＮＡ存在结构上差异，杂交结果也表明杂种Ｆ１的细胞质来源于母本、而恢复基因对不育细胞质ｍｔＤＮＡ的结构不产生直接影响．当用ＣｏｘⅢ为探针杂交时，杂种Ｆ１显示了一特异的同源片段，它不存在于不育系的ｍｔＤＮＡ，而存在于保持系和恢复系的ｍｔＤＮＡ上，这可解释为在不育系和恢复系杂交形成Ｆ１杂种时，恢复系的ｍｔＤ－ＮＡ有可能渗漏到了Ｆ１杂种（Ｐａｔｅｒｎａｌｌｅａｋａｇｅ）．
The polymorphism of mitochondrial DNA in indica type cytoplasmic male sterile line Zhenshan 97A, maintainer line Zhenshan 97B, restorer line Minghui 63 and two F1 hybrids were analyzed. Five sources of mitochondrial DNA were Southern blot analyzed with six mitochondrial DNA probes. When hybridized with CoxⅡ, atpA and 26srRNA as probe, the same hybridization results were found between male sterile line, maintainer line and restorer line, while when CoxⅠ, CoxⅡ, atp6 and 630bpmt DNA fragments were used as probes, There were different hybridization bands between fertile lines, suggesting that the mtDNA of CMS lines and fertile lines were structurally different. The hybridization results also indicated that the cytoplasm of hybrid F1 came from the female parent, whereas the mtDNA of the restorer gene No direct impact. Hybrid F1 showed a specific homologous fragment when hybridized with Cox III as a probe, which is not found in the mtDNA of the CMS but in the mtDNA of the maintainer and restorer lines, The restorer line mtD-NA is likely to leak into the F1 hybrid (Paternalleakage) when it is crossed with the restorer line to form the F1 hybrid.