论文部分内容阅读
目的 :确定 CALM和 AF1 0融合转录在原发性 t( 1 0 ;1 1 )转换的白血病形成和治疗中的作用。方法 :检测 5例原发性 t( 1 0 ;1 1 )转换的白血病患者 CAL M- AF1 0融合转录 ,检测 CALM/AF1 0硫代反义核酸对其白血病细胞体外化疗敏感性和凋亡的作用。结果 :RT-PCR在 5例原发性 t( 1 0 ;1 1 )转换的白血病患者中检测到 5种分子量大小的 AF1 0 - CALM融合转录和 4种分子量大小的 CAL M- AF1 0融合转录。原代 t( 1 0 ;1 1 )转换的白血病细胞对体外化疗敏感性较非 t( 1 0 ;1 1 )转换的显著低 ,而 AS- PS- ODNs增加了其体外化疗敏感性和凋亡率。5μM AS- PS- ODNs处理时有 4例阳性 ,1 0μM和 2 0μM AS- PS- ODNs处理时 5例全阳性 ,而单纯化疗药组和化疗药 + 1 0μM正义硫代核酸 ( S- PS- ODNs)组分别只有 3例。化疗药+ 1 0μM AS- PS- ODNs处理 48h、72 h、96h后 ,细胞凋亡指数分别显著高于单纯化疗药组和化疗药 + 1 0μM S- PS- ODNs组。而单纯化疗药组和化疗药 + 1 0μM S- PS- ODNs组间凋亡指数在对应时间无差别。结论 :CALM- AF1 0与 t( 1 0 ;1 1 )转换白血病的发生密切相关 ,且 t( 1 0 ;1 1 )转换的白血病患者预后较差。
OBJECTIVE: To determine the role of CALM and AF1 0 fusion transcripts in the formation and treatment of primary t (1 0; 1 1) transformed leukemia. METHODS: CAL M-AF1 0 fusion transcripts were detected in 5 primary leukemia patients with t (1 0; 1 1) conversion and the chemosensitivity and apoptosis of CALM / AF1 0 antisense oligodeoxyribonucleic acid effect. RESULTS: Five molecular weight AF1 0 - CALM fusion transcripts and four molecular weight CAL M-AF1 0 fusion transcripts were detected by RT-PCR in five primary t (l0; l1) transformed leukemia patients . Primary (10; 1 1) transformed leukemic cells showed significantly lower in vitro chemosensitivity than non-t (1 0; 1 1) conversions, whereas AS-PS-ODNs increased their chemosensitivity and apoptosis in vitro rate. 4 cases were positive in 5μM AS-PS-ODNs treatment, 5 cases were positive in 10μM and 20μM AS-PS-ODNs treatment, whereas chemotherapy alone group and chemotherapeutic drugs + 10μM S-PS- ODNs) group were only 3 cases. The apoptotic index of the chemotherapeutic drugs + 10μM AS-PS-ODNs treated with 48h, 72h, 96h after chemotherapy were significantly higher than that of chemotherapy alone and chemotherapy alone + 100μM S-PS-ODNs. There was no difference in apoptosis index between chemotherapy alone and chemotherapeutic drugs + 10 μM S-PS-ODNs. CONCLUSION: CALM-AF1 0 is closely related to the occurrence of leukemia with t (1 0; 1 1) transfection, and the prognosis of leukemia with t (1 0; 1 1) conversion is poor.