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目的:研究中国东部地区幽门螺杆菌(H.pylori)cagA、vacA和iceA1毒力基因型的分布状况及其与胃癌的相关性。方法:从52例病人胃黏膜活检组织(31例慢性浅表性胃炎,21例胃癌)中分离培养H.pylori,用PCR方法扩增分离菌株的cagA、iceA1、vacAs,i,m区毒力基因片段,统计并分析上述毒力因素与胃癌发生的相关性。结果:在52例菌株中,cagA、vacAs1/i1/m1、vacAs1/i1/m2和iceA1的阳性率分别是92.3%(48/52),48.1%(25/52),48.1%(25/52),90.4%(47/52);所有的胃癌分离株中均为cagA(+)vacAs1/i1(+)型,vacAm1、vacAm2和iceA1在胃癌组中的阳性率分别是47.6%(10/21),52.4%(11/21),95.2%(20/21),与胃炎组相比,上述基因型的阳性率差异均无显著统计学意义(P>0.05)。结论:cagA、vacAs1/i1/m1、vacAs1/i1/m2和iceA1是中国东部地区H.pylori的优势基因型;cagA、vacAs1、vacAi1和iceA1毒力基因型的存在与胃癌的发生无相关性。
Objective: To study the distribution of cagA, vacA and iceA1 virulence genotypes in Helicobacter pylori (H. pylori) and its relationship with gastric cancer in eastern China. Methods: H.pylori was isolated and cultured from gastric mucosal biopsies of 52 patients (31 cases of chronic superficial gastritis and 21 cases of gastric cancer). The virulence of cagA, iceA1, vacAs, i and m isolates was amplified by PCR Gene fragments, statistics and analysis of the virulence factors and the occurrence of gastric cancer. RESULTS: The positive rates of cagA, vacAs1 / i1 / m1, vacAs1 / i1 / m2 and iceA1 were 92.3% (48/52), 48.1% (25/52) and 48.1% (25/52 ) And 90.4% (47/52) respectively. The positive rates of vacA1, vacAm2 and iceA1 in gastric cancer were cagA (+) vacAs1 / i1 (+) in all gastric cancer isolates, ), 52.4% (11/21) and 95.2% (20/21) respectively. There was no significant difference in the positive rates of these genotypes between the two groups (P> 0.05). Conclusion: cagA, vacAs1 / i1 / m1, vacAs1 / i1 / m2 and iceA1 are the predominant genotypes of H.pylori in eastern China. The genotypes of cagA, vacAs1, vacAi1 and iceA1 have no correlation with the occurrence of gastric cancer.