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以结瘤和非结瘤近等位基因大豆品系为材料,利用砂培方式,分析了不同供氮水平对大豆生长、碳氮代谢、结瘤和固氮的影响。结果表明:供氮水平直接影响大豆生物量,结瘤大豆在N2水平(54 mg尿素·kg-1砂)时生物量达到最大(9.16 g·株-1);非结瘤大豆在N4水平(216 mg尿素·kg-1砂)时生物量达到最大(6.36 g·株-1)。植株体内碳氮含量与生物量的变化趋势相同。此外,结瘤品系N2水平大豆根瘤数最多(205个.株-1),极显著高于除N4以外的其它处理(P<0.01),该水平的共生固氮量最大,为275.92 mg·株-1,且与N3、N4、N5处理间差异极显著(P<0.01)。因此,结瘤和非结瘤大豆对外源氮的需求存在显著差异,该试验条件下的氮阻遏阈值为N2水平,即54 mg尿素·kg-1砂。
Using nodulation and non-nodulation near-allelic soybean lines as materials, the effects of different nitrogen supply levels on soybean growth, carbon and nitrogen metabolism, nodulation and nitrogen fixation were analyzed by sand culture method. The results showed that the nitrogen supply directly affected the biomass of soybean, and the biomass of nodulated soybean reached the maximum (9.16 g · s -1) at N2 level (54 mg urea · kg-1 sand) 216 mg urea · kg-1 sand) reached the highest biomass (6.36 g · plant -1). Plant carbon and nitrogen content and biomass trends the same. In addition, the maximum number of nodules at the N2 level in nodulation lines (205 plants. -1) was significantly higher than that of other treatments except N4 (P <0.01), with the highest symbiotic nitrogen fixation at 275.92 mg · plant- 1, and there was significant difference between N3, N4 and N5 (P <0.01). Therefore, there is a significant difference in the demand of nitrogen for the nodulated and non-nodulated soybeans. Under this experimental condition, the nitrogen inhibition threshold is N2 level, that is, 54 mg of urea · kg-1 of sand.