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1 材料 实验动物:健康雄性Wistar大鼠,体重200～300g,哈医大动物学部提供,实验动物随机分为4组,实验时用2％戊巴比妥钠麻醉,取离体心脏时加肝素抗凝。药品:刺五加注射液(黑龙江省完达山制药厂,红花黄色素:0.3g生药/ml,采用王承颂报道方法自制,再用0.2μm滤膜过滤精制。丙二醛双乙基脲为MDA测定标准品(Sigma.CO.),余为国产分析纯试剂。仪器:采用722分光光度计(上海第三分析仪器厂),MPF-66型荧光分光光度计(美国Perkin-Elmer公司)。2 实验方法 采用Langehdoff无作功非循环式离体心脏灌流
1 Materials: Experimental animals: Healthy male Wistar rats, weighing 200-300 g, provided by Department of Zoology, Harbin Medical University. Experimental animals were randomly divided into 4 groups. During the experiment, anesthesia was performed with 2% pentobarbital sodium, and heparin resistance was taken when the heart was isolated. Condensation. Drugs: Acanthopanax injection (Wandashan Pharmaceutical Factory, Heilongjiang Province, safflower yellow: 0.3g crude drug/ml, self-prepared using Wang Chengsong’s report method, and then refined by 0.2μm filter membrane. Malondialdehyde Diethyl Urea is the MDA standard product (Sigma.CO.), and the rest is domestically produced analytical reagents.. Instrument: 722 spectrophotometer (Shanghai Third Analytical Instruments Factory), MPF-66 fluorescence spectrophotometer (Perkin-Elmer, USA) (2) Experimental method using Langehdoff non-working non-circular isolated heart perfusion