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目的 研究侧脑室给予一氧化氮 (NO)前体L -精氨酸 (L -Arg)对清醒大鼠胃运动及胃电的影响及其作用机制。方法 采用侧脑室微量注射药物、应力传感器引导胃运动及银丝电极同步记录胃电等方法。结果 侧脑室注射 0 .5mg或 1mgL -Arg后胃运动及胃电幅度明显增强 ,胃运动频率无明显变化 ,而静脉注射 0 .5mgL-Arg对胃运动及胃电幅度没有影响 ;事先侧脑室注射 0 .2 5mgNO合酶抑制剂L -硝基精氨酸甲酯 (L -NAME)或切断膈下迷走神经 ,则可完全消除L -Arg对胃运动及胃电的影响 ,但切除腹腔交感神经节后不影响L -Arg的作用。结论 侧脑室给予NO前体物质可使胃运动及胃电增强 ,这种效应系中枢作用 ,并且可能通过迷走神经介导 ,似与交感神经关系不大
Objective To study the effects of L-arginine (L-Arg), a nitric oxide (NO) precursor, in the lateral ventricle on gastric motility and gastric electrical activity in conscious rats and its mechanism. Methods Microinjection of drugs into the lateral ventricle, stress sensors to guide gastric motility and synchronous recording of gastric electrodes with silver electrodes and other methods. Results After intracerebroventricular injection of 0.5 mg or 1 mg L -Arg, gastric motility and gastric electrical amplitude were significantly increased, while gastric motility frequency did not change significantly. Intravenous injection of 0.5 mg L-Arg had no effect on gastric motility and gastric electrical amplitude. 0 .2 5 mg of NO synthase inhibitor, L-NAME, or severance of the subdiaphragmatic vagus nerve completely abolished the effects of L-Arg on gastric motility and gastric electrogastrum, but resected the sympathetic ganglia Does not affect the role of L-Arg. Conclusions Administration of NO precursors to the lateral ventricle can enhance gastric motility and gastric electrical activity. This effect is central and may be mediated through the vagus nerve, and seems to be less related to the sympathetic nerve