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目的：探讨一氧化氮（ＮＯ）及氧自由基（ＯＦＲ）在新生儿缺氧缺血性脑病（ＨＩＥ）中的作用及意义。方法：取正常及ＨＩＥ急性期新生儿的静脉血制备成血浆，采用硝酸还原酶法测定ＮＯ的含量，用化学比色法测定ＭＤＡ的含量及ＳＯＤ的活力，用ＥＬＩＳＡ法测定ＮＳＥ水平。结果：ＨＩＥ急性期新生儿血浆ＮＯ、ＭＤＡ及ＮＳＥ水平较正常对照组新生儿均明显增高（均Ｐ＜０．０１）；而ＳＯＤ活力则明显降低（Ｐ＜０．０１）。结论：ＮＯ和ＯＦＲ与新生儿ＨＩＥ的发病机制有关。
Objective: To investigate the role and significance of nitric oxide (NO) and oxygen free radical (OFR) in neonatal hypoxic-ischemic encephalopathy (HIE). Methods: The plasma of neonates with normal and HIE acute phase was prepared. The content of NO was determined by nitrate reductase method. The content of MDA and the activity of SOD were determined by chemical colorimetry. The level of NSE was determined by ELISA. Results: The levels of plasma NO, MDA and NSE in neonates with HIE in the acute phase were significantly higher than those in the normal control group (all P <0.01), while the activity of SOD was significantly lower (P <0.01). Conclusion: NO and OFR are related to the pathogenesis of neonatal HIE.